Dobrava virus as a new hantavirus

Evidenced by comparative sequence analysis

S. Y. Xiao, G. Diglisic, T. Avsic-Zupanc, James LeDuc

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Dobrava virus, recently isolated from a yellow-neck mouse (Apodemus flavicollis), captured in a northern Slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of PCR-amplified gene segments to be related to previously recognized hantaviruses. To investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (M) genome segment of Dobrava virus was amplified by PCR and the nucleotide sequence determined. Comparing the nucleotide sequence with the same gene region other hantaviruses revealed an overall homology of 41.7%. A phylogenetic tree based on pair-wise sequence similarity clearly showed that Dobrava virus is genetically distinct, and probably represents a new virus in the genus Hantavirus of the family Bunyaviridae.

Original languageEnglish (US)
Pages (from-to)152-155
Number of pages4
JournalJournal of Medical Virology
Volume39
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Hantavirus
Sequence Analysis
Viruses
Bunyaviridae
Hemorrhagic Fever with Renal Syndrome
Murinae
Polymerase Chain Reaction
Serology
Genes
Digestion
Neck
Genome
Enzymes

Keywords

  • Hantaan virus
  • Hemorrhagic fever with renal syndrome
  • Nucleotide sequence
  • RT-PCR
  • Slovenia

ASJC Scopus subject areas

  • Virology

Cite this

Dobrava virus as a new hantavirus : Evidenced by comparative sequence analysis. / Xiao, S. Y.; Diglisic, G.; Avsic-Zupanc, T.; LeDuc, James.

In: Journal of Medical Virology, Vol. 39, No. 2, 1993, p. 152-155.

Research output: Contribution to journalArticle

Xiao, S. Y. ; Diglisic, G. ; Avsic-Zupanc, T. ; LeDuc, James. / Dobrava virus as a new hantavirus : Evidenced by comparative sequence analysis. In: Journal of Medical Virology. 1993 ; Vol. 39, No. 2. pp. 152-155.
@article{a2f4df7170c144b0a5134ea3c15d50c1,
title = "Dobrava virus as a new hantavirus: Evidenced by comparative sequence analysis",
abstract = "Dobrava virus, recently isolated from a yellow-neck mouse (Apodemus flavicollis), captured in a northern Slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of PCR-amplified gene segments to be related to previously recognized hantaviruses. To investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (M) genome segment of Dobrava virus was amplified by PCR and the nucleotide sequence determined. Comparing the nucleotide sequence with the same gene region other hantaviruses revealed an overall homology of 41.7{\%}. A phylogenetic tree based on pair-wise sequence similarity clearly showed that Dobrava virus is genetically distinct, and probably represents a new virus in the genus Hantavirus of the family Bunyaviridae.",
keywords = "Hantaan virus, Hemorrhagic fever with renal syndrome, Nucleotide sequence, RT-PCR, Slovenia",
author = "Xiao, {S. Y.} and G. Diglisic and T. Avsic-Zupanc and James LeDuc",
year = "1993",
doi = "10.1002/jmv.1890390212",
language = "English (US)",
volume = "39",
pages = "152--155",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Dobrava virus as a new hantavirus

T2 - Evidenced by comparative sequence analysis

AU - Xiao, S. Y.

AU - Diglisic, G.

AU - Avsic-Zupanc, T.

AU - LeDuc, James

PY - 1993

Y1 - 1993

N2 - Dobrava virus, recently isolated from a yellow-neck mouse (Apodemus flavicollis), captured in a northern Slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of PCR-amplified gene segments to be related to previously recognized hantaviruses. To investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (M) genome segment of Dobrava virus was amplified by PCR and the nucleotide sequence determined. Comparing the nucleotide sequence with the same gene region other hantaviruses revealed an overall homology of 41.7%. A phylogenetic tree based on pair-wise sequence similarity clearly showed that Dobrava virus is genetically distinct, and probably represents a new virus in the genus Hantavirus of the family Bunyaviridae.

AB - Dobrava virus, recently isolated from a yellow-neck mouse (Apodemus flavicollis), captured in a northern Slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of PCR-amplified gene segments to be related to previously recognized hantaviruses. To investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (M) genome segment of Dobrava virus was amplified by PCR and the nucleotide sequence determined. Comparing the nucleotide sequence with the same gene region other hantaviruses revealed an overall homology of 41.7%. A phylogenetic tree based on pair-wise sequence similarity clearly showed that Dobrava virus is genetically distinct, and probably represents a new virus in the genus Hantavirus of the family Bunyaviridae.

KW - Hantaan virus

KW - Hemorrhagic fever with renal syndrome

KW - Nucleotide sequence

KW - RT-PCR

KW - Slovenia

UR - http://www.scopus.com/inward/record.url?scp=0027408836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027408836&partnerID=8YFLogxK

U2 - 10.1002/jmv.1890390212

DO - 10.1002/jmv.1890390212

M3 - Article

VL - 39

SP - 152

EP - 155

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -