Does preincubation with prostaglandin e1 or prostaglandin e2 enhance oxytocin-induced myometrial contractility in vitro?

Giuseppe Chiossi, Luckey C. Reed, Maged M. Costantine, Gary D.V. Hankins, George R. Saade, Monica Longo

Research output: Contribution to journalArticle

Abstract

Objective To determine if preincubation with prostaglandin E1 (PGE1) and E2 (PGE2) enhances oxytocin-induced myometrial contractility in vitro. Study Design Myometrial strips from 13 women were incubated with PGE1 (10-5 mol/L or 10-6 mol/L), PGE2 (10-5 mol/L or 10-6 mol/L) or solvent before adding cumulative concentrations of oxytocin (10 -10 to 10-6 mol/L). The area under the contraction curve was calculated after addition of each agent. One- and two-way analysis of variance was used for comparison (significance p < 0.05). Results PGE2 10-5 mol/L reduced response to oxytocin 10-9 to 10 -6 mol/L (p < 0.05). PGE2 reduced spontaneous myometrial contractility as compared with PGE1 (p < 0.05). A dose-dependent negative effect of prostaglandins was detected on oxytocin 10-8 mol/L (10 -5 mol/L > 10-6 mol/L; p < 0.05). Conclusion Contrary to the hypothesis, neither PGE1 nor PGE2 enhanced oxytocin-induced myometrial contractility; in fact, PGE2 decreased contractility.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalAmerican Journal of Perinatology
Volume30
Issue number8
DOIs
StatePublished - Jan 1 2013

Keywords

  • organ chamber
  • oxytocin
  • prostaglandin E1
  • prostaglandin E2
  • uterine contractility

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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