Donor site wound protein synthesis correlates with length of acute hospitalization in severely burned children

Demidmaa Tuvdendorj, David L. Chinkes, Xiao Jun Zhang, Asle Aarsland, David N. Herndon

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Autografting of burn wounds results in generation of donor site wounds. Here we measured donor site wound protein fractional synthesis rate (FSR) in a burn pediatric population and showed that FSR increases over time postsurgery and correlates with the length of hospital stay (LOS) normalized for total body surface area (TBSA) burn size. 3.9 ± 1.1 days after the grafting surgery patients participated in a metabolic study consisting of continuous infusion of l-[ring-2H5]-phenylalanine and donor site wound punch biopsies. Donor site wound protein FSR was 10.4 ± 7.5%/day. Wound FSR demonstrated linear correlation with the time postsurgery (p<0.05). Multiple regression analysis showed that LOS/TBSA correlated with donor site wound protein FSR and time postsurgery (p<0.001) and the following equation describes the relationship: estimated LOS/TBSA=(FSR-12.95-1.414 × postsurgery day)/(-17.8). This equation predicted that FSR corrected for the postsurgery day when the metabolic study was conducted accounted for 67% of the variability (r2=0.673) in the LOS/TBSA. Donor site wound protein FSR correlated to LOS/TBSA of burn patients admitted to the intensive care unit. Measurement of protein deposition in regenerating donor site wound using stable isotope technique provides a quantitative measure of wound healing.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalWound Repair and Regeneration
Volume18
Issue number3
DOIs
StatePublished - Jan 1 2010

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ASJC Scopus subject areas

  • Surgery
  • Dermatology

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