Dopamine signaling is required for depolarization-induced slow current in cerebellar Purkinje cells

Yu Shin Kim, Hoon Shin Jung, F. Scott Hall, David J. Linden

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Brief strong depolarization of cerebellar Purkinje cells produces a slow inward cation current. This current, called depolarization-induced slow current (DISC), is triggered by Ca influx in the Purkinje cell and is attenuated by a blocker of vesicular fusion. Previous work in other brain regions, such as the substantia nigra and ventral tegmental area, has shown that dopamine can be released from dendrites to produce paracrine and autocrine signaling. Here,wetest the hypothesis that postsynaptic release of dopamine and autocrine activation of dopamine receptors is involved in DISC. Light immunohistochemistry showed that D3 dopamine receptors, vesicular monoamine transporter type 2 (VMAT2), and dopamine plasma membrane transporters (DATs) were all expressed in cerebellar Purkinje cells. However, their expression was strongest in the gyrus region of cerebellar lobules IX and X. Comparison of DISC across lobules revealed that it was weak in the anterior portions of the cerebellum (lobules II, V, and VI) and strong in lobules IX and X. DISC was blocked by dopamine receptor antagonists (haloperidol, clozapine, eticlopride, and SCH23390). Likewise, DISC was strongly attenuated by inhibitors of VMAT (reserpine and tetrabenazine) and DAT (GBR12909 and rimcazole). These drugs did not produce DISC attenuation through blockade of depolarization-evoked Purkinje cell Ca transients. Purkinje cells in cerebellar slices derived from DAT-null mice expressed DISC, but this DISC ran down at a significantly higher rate than littermate controls. Together, these results suggest that strong Purkinje cell depolarization produces Ca-dependent release of vesicular postsynaptic dopamine that then excites Purkinje cells in an autocrine manner.

Original languageEnglish (US)
Pages (from-to)8530-8538
Number of pages9
JournalJournal of Neuroscience
Volume29
Issue number26
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

Fingerprint

Purkinje Cells
Dopamine
Membrane Transport Proteins
eticlopride
Cell Membrane
Tetrabenazine
Autocrine Communication
Vesicular Monoamine Transport Proteins
Paracrine Communication
Dopamine D3 Receptors
Ventral Tegmental Area
Dopamine Antagonists
Clozapine
Reserpine
Dopamine Receptors
Substantia Nigra
Haloperidol
Dendrites
Cerebellum
Cations

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dopamine signaling is required for depolarization-induced slow current in cerebellar Purkinje cells. / Kim, Yu Shin; Jung, Hoon Shin; Hall, F. Scott; Linden, David J.

In: Journal of Neuroscience, Vol. 29, No. 26, 01.07.2009, p. 8530-8538.

Research output: Contribution to journalArticle

Kim, Yu Shin ; Jung, Hoon Shin ; Hall, F. Scott ; Linden, David J. / Dopamine signaling is required for depolarization-induced slow current in cerebellar Purkinje cells. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 26. pp. 8530-8538.
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