Dopamine suppresses IL-12 p40 production by lipopolysaccharide-stimulated macrophages via a β-adrenoceptor-mediated mechanism

György Haskó, Csaba Szabo, Zoltán H. Németh, Edwin A. Deitch

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In this study, we examined the effect of dopamine on the production of IL-12 p40 by lipopolysaccharide (LPS)-stimulated J774.1 macrophages and mouse peritoneal macrophages. Treatment of J774.1 cells with dopamine (0.01-100 μM) decreased the release of IL-12 p40, in a concentration-dependent manner. The attenuating effect of dopamine on IL-12 p40 production appeared to be pretranslational, because dopamine decreased mRNA accumulation of IL-12 p40. The inhibitory effect of dopamine on IL-12 p40 production by J774.1 macrophages was not mediated by dopamine receptors, because dopamine receptor antagonists were unable to reverse the dopamine-induced suppression of IL-12 p40 production. Since the β-adrenoceptor antagonist propranolol completely prevented the inhibitory effect of dopamine on IL-12 p40 production, the suppressive effect of dopamine on IL-12 p40 production by J774.1 cells is mediated by β-adrenoceptors. In contrast to J774.1 cells, propranolol only partially reversed the inhibitory effect of dopamine on IL-12 production by peritoneal macrophages. Furthermore, dopamine stimulated the production of the anti-inflammatory cytokine IL-10 in both J774.1 cells and peritoneal macrophages. While the stimulatory effect of dopamine on IL-10 production by J774.1 cells was β-adrenoceptor-mediated, dopamine increased IL-10 production by peritoneal macrophages via both β-adrenoceptor-dependent and independent mechanisms. These results indicate that dopamine has multiple anti-inflammatory effects mediated by both β-adrenoceptor dependent and independent mechanisms.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalJournal of Neuroimmunology
Volume122
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Interleukin-12
Adrenergic Receptors
Lipopolysaccharides
Dopamine
Macrophages
Peritoneal Macrophages
Interleukin-10
Propranolol
adjuvant P40
Anti-Inflammatory Agents
Dopamine Antagonists
Dopamine Receptors
Cytokines

Keywords

  • Adrenergic
  • Endotoxin
  • Inflammation
  • Monocyte

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Dopamine suppresses IL-12 p40 production by lipopolysaccharide-stimulated macrophages via a β-adrenoceptor-mediated mechanism. / Haskó, György; Szabo, Csaba; Németh, Zoltán H.; Deitch, Edwin A.

In: Journal of Neuroimmunology, Vol. 122, No. 1-2, 2002, p. 34-39.

Research output: Contribution to journalArticle

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