Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures

R. L. Kohl, W. B. Quay, J. R. Perez‐Polo

    Research output: Contribution to journalArticle

    Abstract

    Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl‐chloride‐reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which (14C)‐labeled internal standard was added to the samples before reaction with (3M)‐labeled dansyl chloride. Values obtained by ion‐exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.

    Original languageEnglish (US)
    Pages (from-to)561-566
    Number of pages6
    JournalJournal of Neuroscience Research
    Volume16
    Issue number3
    DOIs
    StatePublished - 1986

    Keywords

    • amino acids
    • glia
    • neurotransmitter

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience

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