Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation

Attila Bacsi, Leopoldo Aguilera-Aguirre, Bartosz Szczesny, Zsolt Radak, Tapas Hazra, Sanjiv Sur, Xueqing Ba, Istvan Boldogh

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Allergic airway inflammation is characterized by increased expression of pro-inflammatory mediators, inflammatory cell infiltration, mucus hypersecretion, and airway hyperresponsiveness, in parallel with oxidative DNA base and strand damage, whose etiological role is not understood. Our goal was to establish the role of 8-oxoguanine (8-oxoG), a common oxidatively damaged base, and its repair by 8-oxoguanine DNA glycosylase 1 (Ogg1) in allergic airway inflammatory processes. Airway inflammation was induced by intranasally administered ragweed (Ambrosia artemisiifolia) pollen grain extract (RWPE) in sensitized BALB/c mice. We utilized siRNA technology to deplete Ogg1 from airway epithelium; 8-oxoG and DNA strand break levels were quantified by Comet assays. Inflammatory cell infiltration and epithelial methaplasia were determined histologically, mucus and cytokines levels biochemically and enhanced pause was used as the main index of airway hyperresponsiveness. Decreased Ogg1 expression and thereby 8-oxoG repair in the airway epithelium conveyed a lower inflammatory response after RWPE challenge of sensitized mice, as determined by expression of Th2 cytokines, eosinophilia, epithelial methaplasia, and airway hyperresponsiveness. In contrast, 8-oxoG repair in Ogg1-proficient airway epithelium was coupled to an increase in DNA single-strand break (SSB) levels and exacerbation of allergen challenge-dependent inflammation. Decreased expression of the Nei-like glycosylases Neil1 and Neil2 that preferentially excise ring-opened purines and 5-hydroxyuracil, respectively, did not alter the above parameters of allergic immune responses to RWPE. These results show that DNA SSBs formed during Ogg1-mediated repair of 8-oxoG augment antigen-driven allergic immune responses. A transient modulation of OGG1 expression/activity in airway epithelial cells could have clinical benefits.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalDNA Repair
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

DNA Glycosylases
Pneumonia
Down-Regulation
Epithelium
Ambrosia
Mucus
Inflammation
Repair
Epithelial Cells
Cytokines
Single-Stranded DNA Breaks
Purines
DNA Breaks
Comet Assay
DNA
Eosinophilia
Pollen
Infiltration
Allergens
Small Interfering RNA

Keywords

  • 8-oxoG
  • 8-Oxoguanine
  • 8-Oxoguanine DNA glycosylase 1
  • AECs
  • AHR
  • Allergic inflammation
  • AP sites
  • APE1
  • BALF
  • BER
  • CA
  • DNA repair
  • DSB
  • FapyG
  • GSH, GSSG
  • HDCF-DA
  • MEF
  • Neil1/Neil2
  • Neil1/Neil2
  • NOX
  • Ogg1
  • Ogg1
  • Ogg1
  • Oxidative DNA damage
  • Oxidative stress
  • ROS
  • RWPE
  • SSBs

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation. / Bacsi, Attila; Aguilera-Aguirre, Leopoldo; Szczesny, Bartosz; Radak, Zsolt; Hazra, Tapas; Sur, Sanjiv; Ba, Xueqing; Boldogh, Istvan.

In: DNA Repair, Vol. 12, No. 1, 01.01.2013, p. 18-26.

Research output: Contribution to journalArticle

Bacsi, Attila ; Aguilera-Aguirre, Leopoldo ; Szczesny, Bartosz ; Radak, Zsolt ; Hazra, Tapas ; Sur, Sanjiv ; Ba, Xueqing ; Boldogh, Istvan. / Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation. In: DNA Repair. 2013 ; Vol. 12, No. 1. pp. 18-26.
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