Downregulation of Prohormone Convertase-1 by a Phorbol Ester

V. Udupi, Courtney Townsend, Guillermo Gomez, T. Zhang, G. H. Greeley

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acute TPA treatment (1h, 100nM) of a human pancreatic carcinoid cell line (BON) depletes cell contents of chromogranin A (CGA) and pancreastatin (PST), a peptide derived posttranslationally from CGA. Despite removal of TPA, BON cells continue to release CGA in an unregulated fashion whereas PST secretion is reduced substantially. TPA treatment also reduced prohormone convertase-1 (PC-1) protein and increased PC-1 mRNA levels. Together, these findings indicate that the TPA-induced switch from a regulated to unregulated pattern of CGA secretion is accompanied by a decrease in the processing of CGA to PST and a decrease in the active form of a processing enzyme potentially involved in processing CGA to a smaller peptide, PST.

Original languageEnglish (US)
Pages (from-to)495-500
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number2
DOIs
StatePublished - Dec 14 1995

Fingerprint

Proprotein Convertase 1
Chromogranin A
Phorbol Esters
Down-Regulation
Processing
Peptides
Carcinoid Tumor
Cells
Switches
Cell Line
Messenger RNA
pancreastatin
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Downregulation of Prohormone Convertase-1 by a Phorbol Ester. / Udupi, V.; Townsend, Courtney; Gomez, Guillermo; Zhang, T.; Greeley, G. H.

In: Biochemical and Biophysical Research Communications, Vol. 217, No. 2, 14.12.1995, p. 495-500.

Research output: Contribution to journalArticle

Udupi, V. ; Townsend, Courtney ; Gomez, Guillermo ; Zhang, T. ; Greeley, G. H. / Downregulation of Prohormone Convertase-1 by a Phorbol Ester. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 217, No. 2. pp. 495-500.
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