DP reactive antibody in a zero mismatch renal transplant pair

Smita Vaidya, Brant Hilson, Susan Sheldon, Pedro Cano, Marcelo Fernandez-Vina

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

This study describes molecular basis for positive B-cell flow crossmatch in a zero mismatch (0MM) transplant pair. Our end-stage renal disease patient was B-cell flow crossmatch positive with a 0MM deceased donor. DNA from the donor and patient were further typed by a high-resolution method. The patient's sera were tested for anti-HLA reactivity by single antigen bead using a Luminex platform. The patient and donor were found to be HLA identical except for a single DP allele MM (DPB1*0601). As there was no single antigen bead coated with DPB*0601, analysis of the amino acid residues of reactive and nonreactive DPB1 alleles was conducted. The results showed that all reactive alleles carried the amino acid D-E at residue 55 and 56 of DPB1. The MM allele DPB1*0601 also carries the DE 55-56 epitope. We conclude that positive B-cell flow crossmatch was likely the result of single MM in the DP locus.

Original languageEnglish (US)
Pages (from-to)947-949
Number of pages3
JournalHuman Immunology
Volume68
Issue number12
DOIs
StatePublished - Dec 2007

Keywords

  • Amino acid residue analysis
  • Flow crossmatch
  • Luminex platform

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Vaidya, S., Hilson, B., Sheldon, S., Cano, P., & Fernandez-Vina, M. (2007). DP reactive antibody in a zero mismatch renal transplant pair. Human Immunology, 68(12), 947-949. https://doi.org/10.1016/j.humimm.2007.10.013