Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line

Pawel Goluszko, Vsevolod Popov, Rangaraj Selvarangan, Stella Nowicki, Tuan Pham, Bogdan J. Nowicki

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Escherichia coli Dr adhesin and decay-accelerating factor (DAF) receptor-mediated interaction was proposed as the mechanism of ascending urinary tract infection (UTI) and chronic interstitial nephritis. This report provides novel evidence for Dr fimbriae operon-mediated invasive capacity of Dr + E. coli. Insertional mutants draE, draC, and draB, and adherent draD and UV-inactivated BN406 were unable to enter HeLa cells. Complementation of the dra mutation restored invasiveness. Internalization was inhibited by anti-Dr fimbriae IgG (100%), anti-SCR-3 domain of DAF (75%), and nocodazole (95%). Increased receptor-ligand density occurred at the site of internalization. Internalized Dr + E. coli did not significantly multiply in the HeLa cell line. Accordingly, the dra operon and DAF were required for microtubule- dependent internalization of E. coli to HeLa cells. The relatively low invasion and multiplication rates of Dr + E. coli may hypothetically contribute to the postattachment steps of ascending UTI and chronic renal infection.

Original languageEnglish (US)
Pages (from-to)158-167
Number of pages10
JournalJournal of Infectious Diseases
Volume176
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Uropathogenic Escherichia coli
CD55 Antigens
Operon
HeLa Cells
Microtubules
Epithelial Cells
Escherichia coli
Cell Line
Urinary Tract Infections
Escherichia coli Adhesins
Nocodazole
Interstitial Nephritis
Ligands
Kidney
Mutation
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line. / Goluszko, Pawel; Popov, Vsevolod; Selvarangan, Rangaraj; Nowicki, Stella; Pham, Tuan; Nowicki, Bogdan J.

In: Journal of Infectious Diseases, Vol. 176, No. 1, 1997, p. 158-167.

Research output: Contribution to journalArticle

Goluszko, Pawel ; Popov, Vsevolod ; Selvarangan, Rangaraj ; Nowicki, Stella ; Pham, Tuan ; Nowicki, Bogdan J. / Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line. In: Journal of Infectious Diseases. 1997 ; Vol. 176, No. 1. pp. 158-167.
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