Administration of the potent antineoplastic agent, 5-azacytidine (1-β-D-ribofuranosyl-4-amino-s-triazine-2(1H)-one), to mice causes a marked reduction of the 5-methylcytidine content of liver transfer RNA. This effect is dose-dependent and specific for the methylation of the 5-position of cytidine; the drug has no effect on 3-methylcytidine, 4-acetylcytidine, 5-methyluridine, dihydrouridine, pseudouridine, and modified purines. The mechanism of this effect has not yet been elucidated but probably involves the selective inhibition of 5-methylcytosine methyltransferase(s) by 5-azacytidine itself or its metabolites. The results presented show that the modified constituents of nucleic acids provide potential targets for chemotherapeutic agents.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 23 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology