Drug effects on nucleic acid modification. I. A specific effect of 5-azacytidine on mammalian transfer RNA methylation invivo

Leejane Wang Lu, Grace Han Chiang, Daniel Medina, Kurt Randerath

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Administration of the potent antineoplastic agent, 5-azacytidine (1-β-D-ribofuranosyl-4-amino-s-triazine-2(1H)-one), to mice causes a marked reduction of the 5-methylcytidine content of liver transfer RNA. This effect is dose-dependent and specific for the methylation of the 5-position of cytidine; the drug has no effect on 3-methylcytidine, 4-acetylcytidine, 5-methyluridine, dihydrouridine, pseudouridine, and modified purines. The mechanism of this effect has not yet been elucidated but probably involves the selective inhibition of 5-methylcytosine methyltransferase(s) by 5-azacytidine itself or its metabolites. The results presented show that the modified constituents of nucleic acids provide potential targets for chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)1094-1101
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume68
Issue number4
DOIs
StatePublished - Feb 23 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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