Drug effects on nucleic acid modification. I. A specific effect of 5-azacytidine on mammalian transfer RNA methylation invivo

Leejane Lu, Grace Han Chiang, Daniel Medina, Kurt Randerath

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Administration of the potent antineoplastic agent, 5-azacytidine (1-β-D-ribofuranosyl-4-amino-s-triazine-2(1H)-one), to mice causes a marked reduction of the 5-methylcytidine content of liver transfer RNA. This effect is dose-dependent and specific for the methylation of the 5-position of cytidine; the drug has no effect on 3-methylcytidine, 4-acetylcytidine, 5-methyluridine, dihydrouridine, pseudouridine, and modified purines. The mechanism of this effect has not yet been elucidated but probably involves the selective inhibition of 5-methylcytosine methyltransferase(s) by 5-azacytidine itself or its metabolites. The results presented show that the modified constituents of nucleic acids provide potential targets for chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)1094-1101
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume68
Issue number4
DOIs
StatePublished - Feb 23 1976
Externally publishedYes

Fingerprint

Azacitidine
Methylation
Transfer RNA
Nucleic Acids
Pseudouridine
5-Methylcytosine
Cytidine
Triazines
Purines
Methyltransferases
Metabolites
Pharmaceutical Preparations
Liver
Antineoplastic Agents
ribothymidine
N-acetylcytidine
3-methylcytidine
5-methylcytidine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Drug effects on nucleic acid modification. I. A specific effect of 5-azacytidine on mammalian transfer RNA methylation invivo. / Lu, Leejane; Chiang, Grace Han; Medina, Daniel; Randerath, Kurt.

In: Biochemical and Biophysical Research Communications, Vol. 68, No. 4, 23.02.1976, p. 1094-1101.

Research output: Contribution to journalArticle

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