Dual action of arachidonic acid on calcium mobilization in avian granulosa cells

F. Hertelendy, M. Molnár, M. Jamaluddin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 μM) AA released 35% 45Ca from the endoplasmic reticulum (ER), whereas at higher concentrations (ED50 16 μM) it stimulated 45Ca efflux from mitochondria. These effects of AA were mimicked at 10-20 times lower concentration by the calcium ionophore A23187. Inositol 1,4,5-trisphosphate (IP3) also stimulated 45Ca efflux from the ER, with a markedly lower potency than AA (ED50 6.2 μM), as well as exhibiting a biphasic response. Heparin abolished the effect of IP3 and luteinizing hormone (LH), but it had no influence on AA-promoted 45Ca efflux. Moreover, the actions of IP3 and AA were additive, indicating that AA and IP3 access different Ca pools in the ER by different mechanisms. Several other unsaturated fatty acids also stimulated 45Ca mobilization from both ER and mitochondria but, with the exception of eicosapentaenoic acid, were significantly less effective than AA. It is concluded that free AA, at submicromolar concentrations that might be viewed as physiological, is a potent calcium mobilizing agent and thus may play an important role in signal transduction in avian granulosa cells, akin to that of IP3. At high (> 10 μM) concentrations AA removes Ca2+ from the mitochondria, an action that may be responsible for its reported inhibitory effects on steroidogenesis and other cellular functions.

Original languageEnglish (US)
Pages (from-to)173-181
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume83
Issue number2-3
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Granulosa Cells
Arachidonic Acid
Calcium
Endoplasmic Reticulum
Mitochondria
Digitonin
Signal transduction
Inositol 1,4,5-Trisphosphate
Eicosapentaenoic Acid
Calcium Ionophores
Calcimycin
Luteinizing Hormone
Unsaturated Fatty Acids
Heparin
Signal Transduction

Keywords

  • Arachidonic acid
  • Ca mobilization
  • Granulosa cell
  • Ovary, hen
  • Signal transduction

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dual action of arachidonic acid on calcium mobilization in avian granulosa cells. / Hertelendy, F.; Molnár, M.; Jamaluddin, M.

In: Molecular and Cellular Endocrinology, Vol. 83, No. 2-3, 1992, p. 173-181.

Research output: Contribution to journalArticle

Hertelendy, F. ; Molnár, M. ; Jamaluddin, M. / Dual action of arachidonic acid on calcium mobilization in avian granulosa cells. In: Molecular and Cellular Endocrinology. 1992 ; Vol. 83, No. 2-3. pp. 173-181.
@article{b0ac8b984e9b427290e578c4a10f2738,
title = "Dual action of arachidonic acid on calcium mobilization in avian granulosa cells",
abstract = "The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 μM) AA released 35{\%} 45Ca from the endoplasmic reticulum (ER), whereas at higher concentrations (ED50 16 μM) it stimulated 45Ca efflux from mitochondria. These effects of AA were mimicked at 10-20 times lower concentration by the calcium ionophore A23187. Inositol 1,4,5-trisphosphate (IP3) also stimulated 45Ca efflux from the ER, with a markedly lower potency than AA (ED50 6.2 μM), as well as exhibiting a biphasic response. Heparin abolished the effect of IP3 and luteinizing hormone (LH), but it had no influence on AA-promoted 45Ca efflux. Moreover, the actions of IP3 and AA were additive, indicating that AA and IP3 access different Ca pools in the ER by different mechanisms. Several other unsaturated fatty acids also stimulated 45Ca mobilization from both ER and mitochondria but, with the exception of eicosapentaenoic acid, were significantly less effective than AA. It is concluded that free AA, at submicromolar concentrations that might be viewed as physiological, is a potent calcium mobilizing agent and thus may play an important role in signal transduction in avian granulosa cells, akin to that of IP3. At high (> 10 μM) concentrations AA removes Ca2+ from the mitochondria, an action that may be responsible for its reported inhibitory effects on steroidogenesis and other cellular functions.",
keywords = "Arachidonic acid, Ca mobilization, Granulosa cell, Ovary, hen, Signal transduction",
author = "F. Hertelendy and M. Moln{\'a}r and M. Jamaluddin",
year = "1992",
doi = "10.1016/0303-7207(92)90157-2",
language = "English (US)",
volume = "83",
pages = "173--181",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Dual action of arachidonic acid on calcium mobilization in avian granulosa cells

AU - Hertelendy, F.

AU - Molnár, M.

AU - Jamaluddin, M.

PY - 1992

Y1 - 1992

N2 - The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 μM) AA released 35% 45Ca from the endoplasmic reticulum (ER), whereas at higher concentrations (ED50 16 μM) it stimulated 45Ca efflux from mitochondria. These effects of AA were mimicked at 10-20 times lower concentration by the calcium ionophore A23187. Inositol 1,4,5-trisphosphate (IP3) also stimulated 45Ca efflux from the ER, with a markedly lower potency than AA (ED50 6.2 μM), as well as exhibiting a biphasic response. Heparin abolished the effect of IP3 and luteinizing hormone (LH), but it had no influence on AA-promoted 45Ca efflux. Moreover, the actions of IP3 and AA were additive, indicating that AA and IP3 access different Ca pools in the ER by different mechanisms. Several other unsaturated fatty acids also stimulated 45Ca mobilization from both ER and mitochondria but, with the exception of eicosapentaenoic acid, were significantly less effective than AA. It is concluded that free AA, at submicromolar concentrations that might be viewed as physiological, is a potent calcium mobilizing agent and thus may play an important role in signal transduction in avian granulosa cells, akin to that of IP3. At high (> 10 μM) concentrations AA removes Ca2+ from the mitochondria, an action that may be responsible for its reported inhibitory effects on steroidogenesis and other cellular functions.

AB - The primary aim of this study was to evaluate the effects of arachidonic acid (AA) on calcium mobilization from intracellular compartments in digitonin-permeabilized granulosa cells isolated from the largest preovulatory follicles of laying hens. At low concentrations (ED50 0.2 μM) AA released 35% 45Ca from the endoplasmic reticulum (ER), whereas at higher concentrations (ED50 16 μM) it stimulated 45Ca efflux from mitochondria. These effects of AA were mimicked at 10-20 times lower concentration by the calcium ionophore A23187. Inositol 1,4,5-trisphosphate (IP3) also stimulated 45Ca efflux from the ER, with a markedly lower potency than AA (ED50 6.2 μM), as well as exhibiting a biphasic response. Heparin abolished the effect of IP3 and luteinizing hormone (LH), but it had no influence on AA-promoted 45Ca efflux. Moreover, the actions of IP3 and AA were additive, indicating that AA and IP3 access different Ca pools in the ER by different mechanisms. Several other unsaturated fatty acids also stimulated 45Ca mobilization from both ER and mitochondria but, with the exception of eicosapentaenoic acid, were significantly less effective than AA. It is concluded that free AA, at submicromolar concentrations that might be viewed as physiological, is a potent calcium mobilizing agent and thus may play an important role in signal transduction in avian granulosa cells, akin to that of IP3. At high (> 10 μM) concentrations AA removes Ca2+ from the mitochondria, an action that may be responsible for its reported inhibitory effects on steroidogenesis and other cellular functions.

KW - Arachidonic acid

KW - Ca mobilization

KW - Granulosa cell

KW - Ovary, hen

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=0026570552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026570552&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(92)90157-2

DO - 10.1016/0303-7207(92)90157-2

M3 - Article

C2 - 1547910

AN - SCOPUS:0026570552

VL - 83

SP - 173

EP - 181

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 2-3

ER -