Dual functions of rift valley fever virus NSs protein

Inhibition of host mRNA transcription and post-transcriptional downregulation of protein kinase PKR

Tetsuro Ikegami, Krishna Narayanan, Sungyong Won, Wataru Kamitani, C. J. Peters, Shinji Makino

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Rift Valley fever virus (RVFV), which belongs to the genus Phlebovirus, family Bunyaviridae, is a negative-stranded RNA virus carrying a single-stranded, tripartite RNA genome. RVFV is an important zoonotic pathogen transmitted by mosquitoes and causes large outbreaks among ruminants and humans in Africa and the Arabian Peninsula. Human patients develop an acute febrile illness, followed by a fatal hemorrhagic fever, encephalitis, or ocular diseases. A viral nonstructural protein, NSs, is a major viral virulence factor. Past studies showed that NSs suppresses the transcription of host mRNAs, including interferon-β mRNAs. Here we demonstrated that the NSs protein induced post-transcriptional downregulation of dsRNA-dependent protein kinase (PKR), to prevent phosphorylation of eIF2α and promoted viral translation in infected cells. These two biological activities of the NSs most probably have a synergistic effect in suppressing host innate immune functions and facilitate efficient viral replication in infected mammalian hosts.

Original languageEnglish (US)
JournalAnnals of the New York Academy of Sciences
Volume1171
Issue numberSUPPL. 1
DOIs
StatePublished - Sep 2009

Fingerprint

Rift Valley fever virus
eIF-2 Kinase
Transcription
Viruses
Phlebovirus
Fever
Viral Nonstructural Proteins
Down-Regulation
Bunyaviridae
Messenger RNA
Eye Diseases
RNA Viruses
Zoonoses
Ruminants
Virulence Factors
Encephalitis
Culicidae
RNA
Interferons
Disease Outbreaks

Keywords

  • interferon
  • NSs protein
  • PKR
  • Rift Valley fever virus
  • transcriptional suppression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dual functions of rift valley fever virus NSs protein : Inhibition of host mRNA transcription and post-transcriptional downregulation of protein kinase PKR. / Ikegami, Tetsuro; Narayanan, Krishna; Won, Sungyong; Kamitani, Wataru; Peters, C. J.; Makino, Shinji.

In: Annals of the New York Academy of Sciences, Vol. 1171, No. SUPPL. 1, 09.2009.

Research output: Contribution to journalArticle

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