Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2

Xiuhong Feng, Ricardo Oropeza, Linda Kenney

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Expression of genes located on Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection in mice. This region encodes a type III secretion system, secreted effectors and the two-component regulatory system SsrA/B (also referred to as SpiR), as well as additional uncharacterized genes. In the present work, we demonstrate that phospho-OmpR (OmpR-P) functions as an activator at the spiC-ssrA/B locus. There are two promoters at spiR; one is upstream of ssrA and the other upstream of ssrB. Our results indicate that, in contrast to many two-component regulatory systems, regulation of the sensor kinase SsrA appears to be uncoupled and distinct from regulation of the response regulator SsrB. OmpR regulation of ssrA/B is one of only a few examples known in which a two-component response regulator directly regulates the expression of another two-component regulatory system.

Original languageEnglish (US)
Pages (from-to)1131-1143
Number of pages13
JournalMolecular Microbiology
Volume48
Issue number4
DOIs
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Activator Appliances
Genomic Islands
Salmonella
Phosphotransferases
Gene Expression
Infection
Genes
Type III Secretion Systems

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2. / Feng, Xiuhong; Oropeza, Ricardo; Kenney, Linda.

In: Molecular Microbiology, Vol. 48, No. 4, 01.05.2003, p. 1131-1143.

Research output: Contribution to journalArticle

@article{a98a1ea0b04b49be8505574da5dc9497,
title = "Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2",
abstract = "Expression of genes located on Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection in mice. This region encodes a type III secretion system, secreted effectors and the two-component regulatory system SsrA/B (also referred to as SpiR), as well as additional uncharacterized genes. In the present work, we demonstrate that phospho-OmpR (OmpR-P) functions as an activator at the spiC-ssrA/B locus. There are two promoters at spiR; one is upstream of ssrA and the other upstream of ssrB. Our results indicate that, in contrast to many two-component regulatory systems, regulation of the sensor kinase SsrA appears to be uncoupled and distinct from regulation of the response regulator SsrB. OmpR regulation of ssrA/B is one of only a few examples known in which a two-component response regulator directly regulates the expression of another two-component regulatory system.",
author = "Xiuhong Feng and Ricardo Oropeza and Linda Kenney",
year = "2003",
month = "5",
day = "1",
doi = "10.1046/j.1365-2958.2003.03502.x",
language = "English (US)",
volume = "48",
pages = "1131--1143",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2

AU - Feng, Xiuhong

AU - Oropeza, Ricardo

AU - Kenney, Linda

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Expression of genes located on Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection in mice. This region encodes a type III secretion system, secreted effectors and the two-component regulatory system SsrA/B (also referred to as SpiR), as well as additional uncharacterized genes. In the present work, we demonstrate that phospho-OmpR (OmpR-P) functions as an activator at the spiC-ssrA/B locus. There are two promoters at spiR; one is upstream of ssrA and the other upstream of ssrB. Our results indicate that, in contrast to many two-component regulatory systems, regulation of the sensor kinase SsrA appears to be uncoupled and distinct from regulation of the response regulator SsrB. OmpR regulation of ssrA/B is one of only a few examples known in which a two-component response regulator directly regulates the expression of another two-component regulatory system.

AB - Expression of genes located on Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection in mice. This region encodes a type III secretion system, secreted effectors and the two-component regulatory system SsrA/B (also referred to as SpiR), as well as additional uncharacterized genes. In the present work, we demonstrate that phospho-OmpR (OmpR-P) functions as an activator at the spiC-ssrA/B locus. There are two promoters at spiR; one is upstream of ssrA and the other upstream of ssrB. Our results indicate that, in contrast to many two-component regulatory systems, regulation of the sensor kinase SsrA appears to be uncoupled and distinct from regulation of the response regulator SsrB. OmpR regulation of ssrA/B is one of only a few examples known in which a two-component response regulator directly regulates the expression of another two-component regulatory system.

UR - http://www.scopus.com/inward/record.url?scp=0038354822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038354822&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2958.2003.03502.x

DO - 10.1046/j.1365-2958.2003.03502.x

M3 - Article

VL - 48

SP - 1131

EP - 1143

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 4

ER -