Dual requirement for the yeast MMS19 gene in DNA repair and RNA polymerase II transcription

Scott Lauder, Michael Bankmann, Sami N. Guzder, Patrick Sung, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Genetic and biochemical studies of Saccharomyces cerevisiae have indicated the involvement of a large number of protein factors in nucleotide excision repair (NER) of UV-damaged DNA. However, how MMS19 affects this process has remained unclear. Here, we report on the isolation of the MMS19 gene and the determination of its role in NER and other cellular processes. Genetic and biochemical evidence indicates that besides its function in NER, MMS19 also affects RNA polymerase II (Pol II) transcription, mms19Δ cells do not grow at 37°C, and mutant extract exhibits a thermolabile defect in Pol II transcription. Thus, Mms19 protein resembles TFIIH in that it is required for both transcription and DNA repair. However, addition of purified Mms19 protein does not alleviate the transcriptional defect of the mms19Δ extract, nor does it stimulate the incision of UV-damaged DNA reconstituted from purified proteins. Interestingly, addition of purified TFIIH corrects the transcriptional defect of the mms19Δ extract. Mms19 is, however, not a component of TFIIH or of Pol II holoenzyme. These and other results suggest that Mms19 affects NER and transcription by influencing the activity of TFIIH as an upstream regulatory element. It is proposed that mutations in the human MMS19 counterpart could result in syndromes in which both NER and transcription are affected.

Original languageEnglish (US)
Pages (from-to)6783-6793
Number of pages11
JournalMolecular and Cellular Biology
Volume16
Issue number12
StatePublished - 1996

Fingerprint

DNA Polymerase II
RNA Polymerase II
DNA Repair
Yeasts
Genes
Molecular Biology
Proteins
Holoenzymes
DNA
Saccharomyces cerevisiae
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dual requirement for the yeast MMS19 gene in DNA repair and RNA polymerase II transcription. / Lauder, Scott; Bankmann, Michael; Guzder, Sami N.; Sung, Patrick; Prakash, Louise; Prakash, Satya.

In: Molecular and Cellular Biology, Vol. 16, No. 12, 1996, p. 6783-6793.

Research output: Contribution to journalArticle

Lauder, Scott ; Bankmann, Michael ; Guzder, Sami N. ; Sung, Patrick ; Prakash, Louise ; Prakash, Satya. / Dual requirement for the yeast MMS19 gene in DNA repair and RNA polymerase II transcription. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 12. pp. 6783-6793.
@article{39cd89ffb7ce4d1d97e016d96e0b4d67,
title = "Dual requirement for the yeast MMS19 gene in DNA repair and RNA polymerase II transcription",
abstract = "Genetic and biochemical studies of Saccharomyces cerevisiae have indicated the involvement of a large number of protein factors in nucleotide excision repair (NER) of UV-damaged DNA. However, how MMS19 affects this process has remained unclear. Here, we report on the isolation of the MMS19 gene and the determination of its role in NER and other cellular processes. Genetic and biochemical evidence indicates that besides its function in NER, MMS19 also affects RNA polymerase II (Pol II) transcription, mms19Δ cells do not grow at 37°C, and mutant extract exhibits a thermolabile defect in Pol II transcription. Thus, Mms19 protein resembles TFIIH in that it is required for both transcription and DNA repair. However, addition of purified Mms19 protein does not alleviate the transcriptional defect of the mms19Δ extract, nor does it stimulate the incision of UV-damaged DNA reconstituted from purified proteins. Interestingly, addition of purified TFIIH corrects the transcriptional defect of the mms19Δ extract. Mms19 is, however, not a component of TFIIH or of Pol II holoenzyme. These and other results suggest that Mms19 affects NER and transcription by influencing the activity of TFIIH as an upstream regulatory element. It is proposed that mutations in the human MMS19 counterpart could result in syndromes in which both NER and transcription are affected.",
author = "Scott Lauder and Michael Bankmann and Guzder, {Sami N.} and Patrick Sung and Louise Prakash and Satya Prakash",
year = "1996",
language = "English (US)",
volume = "16",
pages = "6783--6793",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Dual requirement for the yeast MMS19 gene in DNA repair and RNA polymerase II transcription

AU - Lauder, Scott

AU - Bankmann, Michael

AU - Guzder, Sami N.

AU - Sung, Patrick

AU - Prakash, Louise

AU - Prakash, Satya

PY - 1996

Y1 - 1996

N2 - Genetic and biochemical studies of Saccharomyces cerevisiae have indicated the involvement of a large number of protein factors in nucleotide excision repair (NER) of UV-damaged DNA. However, how MMS19 affects this process has remained unclear. Here, we report on the isolation of the MMS19 gene and the determination of its role in NER and other cellular processes. Genetic and biochemical evidence indicates that besides its function in NER, MMS19 also affects RNA polymerase II (Pol II) transcription, mms19Δ cells do not grow at 37°C, and mutant extract exhibits a thermolabile defect in Pol II transcription. Thus, Mms19 protein resembles TFIIH in that it is required for both transcription and DNA repair. However, addition of purified Mms19 protein does not alleviate the transcriptional defect of the mms19Δ extract, nor does it stimulate the incision of UV-damaged DNA reconstituted from purified proteins. Interestingly, addition of purified TFIIH corrects the transcriptional defect of the mms19Δ extract. Mms19 is, however, not a component of TFIIH or of Pol II holoenzyme. These and other results suggest that Mms19 affects NER and transcription by influencing the activity of TFIIH as an upstream regulatory element. It is proposed that mutations in the human MMS19 counterpart could result in syndromes in which both NER and transcription are affected.

AB - Genetic and biochemical studies of Saccharomyces cerevisiae have indicated the involvement of a large number of protein factors in nucleotide excision repair (NER) of UV-damaged DNA. However, how MMS19 affects this process has remained unclear. Here, we report on the isolation of the MMS19 gene and the determination of its role in NER and other cellular processes. Genetic and biochemical evidence indicates that besides its function in NER, MMS19 also affects RNA polymerase II (Pol II) transcription, mms19Δ cells do not grow at 37°C, and mutant extract exhibits a thermolabile defect in Pol II transcription. Thus, Mms19 protein resembles TFIIH in that it is required for both transcription and DNA repair. However, addition of purified Mms19 protein does not alleviate the transcriptional defect of the mms19Δ extract, nor does it stimulate the incision of UV-damaged DNA reconstituted from purified proteins. Interestingly, addition of purified TFIIH corrects the transcriptional defect of the mms19Δ extract. Mms19 is, however, not a component of TFIIH or of Pol II holoenzyme. These and other results suggest that Mms19 affects NER and transcription by influencing the activity of TFIIH as an upstream regulatory element. It is proposed that mutations in the human MMS19 counterpart could result in syndromes in which both NER and transcription are affected.

UR - http://www.scopus.com/inward/record.url?scp=0029910712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029910712&partnerID=8YFLogxK

M3 - Article

C2 - 8943333

AN - SCOPUS:0029910712

VL - 16

SP - 6783

EP - 6793

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 12

ER -