Dunning rat prostate adenocarcinomas and alternative splicing reporters: Powerful tools to study epithelial plasticity in prostate tumors in vivo

Sebastian Oltean, Phillip G. Febbo, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions since these cells recapitulate gene expression profiles observed during human prostate cancer progression. In this manuscript we also present the development of two new tools to study the epithelial transitions by imaging alternative splicing decisions: a bichromatic fluorescence reporter to evaluate epithelial transitions in culture and in vivo, and a luciferase reporter to visualize the distribution of mesenchymal epithelial transitions in vivo.

Original languageEnglish (US)
Pages (from-to)611-619
Number of pages9
JournalClinical and Experimental Metastasis
Volume25
Issue number6
DOIs
StatePublished - Oct 2008
Externally publishedYes

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Epithelial-Mesenchymal Transition
Alternative Splicing
Prostate
Adenocarcinoma
Luciferases
Transcriptome
Neoplasms
Prostatic Neoplasms
Fluorescence

Keywords

  • Alternative splicing
  • AT3
  • DT
  • Dunning
  • EMT
  • FGFR2
  • Imaging
  • MET

ASJC Scopus subject areas

  • Cancer Research

Cite this

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abstract = "Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions since these cells recapitulate gene expression profiles observed during human prostate cancer progression. In this manuscript we also present the development of two new tools to study the epithelial transitions by imaging alternative splicing decisions: a bichromatic fluorescence reporter to evaluate epithelial transitions in culture and in vivo, and a luciferase reporter to visualize the distribution of mesenchymal epithelial transitions in vivo.",
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AU - Febbo, Phillip G.

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