Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues

Grady E. Carlson, Eric W. Martin, Venktesh S. Shirure, Ramiro Malgor, Vicente Resto, Douglas J. Goetz, Monica M. Burdick

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional Lselectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional Lselectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue.

Original languageEnglish (US)
Article numbere0173747
JournalPLoS One
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

L-Selectin
Functional analysis
tissue analysis
colorectal neoplasms
Colonic Neoplasms
Tissue
Ligands
Selectins
ligands
tissues
Circulating Neoplastic Cells
hemodynamics
metastasis
Hemodynamics
probes (equipment)
shear stress
colon
Shear flow
adhesion
Microspheres

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Carlson, G. E., Martin, E. W., Shirure, V. S., Malgor, R., Resto, V., Goetz, D. J., & Burdick, M. M. (2017). Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues. PLoS One, 12(3), [e0173747]. https://doi.org/10.1371/journal.pone.0173747

Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues. / Carlson, Grady E.; Martin, Eric W.; Shirure, Venktesh S.; Malgor, Ramiro; Resto, Vicente; Goetz, Douglas J.; Burdick, Monica M.

In: PLoS One, Vol. 12, No. 3, e0173747, 01.03.2017.

Research output: Contribution to journalArticle

Carlson, GE, Martin, EW, Shirure, VS, Malgor, R, Resto, V, Goetz, DJ & Burdick, MM 2017, 'Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues', PLoS One, vol. 12, no. 3, e0173747. https://doi.org/10.1371/journal.pone.0173747
Carlson, Grady E. ; Martin, Eric W. ; Shirure, Venktesh S. ; Malgor, Ramiro ; Resto, Vicente ; Goetz, Douglas J. ; Burdick, Monica M. / Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues. In: PLoS One. 2017 ; Vol. 12, No. 3.
@article{48108d5732b04858bf0df077c83cbdc0,
title = "Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues",
abstract = "A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional Lselectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional Lselectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue.",
author = "Carlson, {Grady E.} and Martin, {Eric W.} and Shirure, {Venktesh S.} and Ramiro Malgor and Vicente Resto and Goetz, {Douglas J.} and Burdick, {Monica M.}",
year = "2017",
month = "3",
day = "1",
doi = "10.1371/journal.pone.0173747",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues

AU - Carlson, Grady E.

AU - Martin, Eric W.

AU - Shirure, Venktesh S.

AU - Malgor, Ramiro

AU - Resto, Vicente

AU - Goetz, Douglas J.

AU - Burdick, Monica M.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional Lselectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional Lselectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue.

AB - A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional Lselectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional Lselectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue.

UR - http://www.scopus.com/inward/record.url?scp=85015158975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015158975&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0173747

DO - 10.1371/journal.pone.0173747

M3 - Article

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e0173747

ER -