Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues

Eric W. Martin, Ramiro Malgor, Vicente Resto, Douglas J. Goetz, Monica M. Burdick

Research output: Contribution to journalArticle

Abstract

Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tissue may serve as valuable assays. Presented herein is an innovative method for detecting functional selectin ligands expressed on human tissue that uses a dynamic approach, which allows for control over the force applied to the bonds between the probe and target molecules. This new method of tissue interrogation, known as dynamic biochemical tissue analysis (DBTA), involves the perfusion of molecular probe-coated microspheres over tissues. DBTA using selectin-coated probes is able to detect functional selectin ligands expressed on tissue from multiple cancer types at both primary and metastatic sites.

Original languageEnglish (US)
Article number8511
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Selectins
Ligands
Neoplasms
Molecular Probes
Microspheres
Cell Adhesion
Endothelium
Leukocytes
Blood Platelets
Perfusion
Neoplasm Metastasis

ASJC Scopus subject areas

  • General

Cite this

Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues. / Martin, Eric W.; Malgor, Ramiro; Resto, Vicente; Goetz, Douglas J.; Burdick, Monica M.

In: Scientific reports, Vol. 9, No. 1, 8511, 01.12.2019.

Research output: Contribution to journalArticle

Martin, Eric W. ; Malgor, Ramiro ; Resto, Vicente ; Goetz, Douglas J. ; Burdick, Monica M. / Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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