@article{0e8b0136eb7c4c0291cfc2ec9440d820,
title = "Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues",
abstract = "Cell adhesion mediated by selectins (expressed by activated endothelium, activated platelets, and leukocytes) binding to their resepective selectin ligands (expressed by cancer cells) may be involved in metastasis. Therefore, methods of characterizing selectin ligands expressed on human tissue may serve as valuable assays. Presented herein is an innovative method for detecting functional selectin ligands expressed on human tissue that uses a dynamic approach, which allows for control over the force applied to the bonds between the probe and target molecules. This new method of tissue interrogation, known as dynamic biochemical tissue analysis (DBTA), involves the perfusion of molecular probe-coated microspheres over tissues. DBTA using selectin-coated probes is able to detect functional selectin ligands expressed on tissue from multiple cancer types at both primary and metastatic sites.",
author = "Martin, {Eric W.} and Ramiro Malgor and Resto, {Vicente A.} and Goetz, {Douglas J.} and Burdick, {Monica M.}",
note = "Funding Information: The authors would like to thank Dr. David Tees of Ohio University{\textquoteright}s Department of Physics & Astronomy, Christian Showalter of Ohio University{\textquoteright}s Molecular and Cellular Biology Program, and Dr. Venktesh Shirure, Dr. Grady Carlson, Kouree Chesser, Tom Riggs, and Carrie Linscott from Ohio University{\textquoteright}s Department of Chemical and Biomolecular Engineering for helpful discussions, critical review of the manuscript, and administrative support. This work was supported by Ohio University (Student Enhancement Award to E.W.M.), the National Institutes of Health (R15CA161830-01 to M.M.B.), and the National Science Foundation (MRI Award 1039869 to D.J.G. and M.M.B.). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-44838-4",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}