Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis

Benedicte Saulier Le Dréan, Andrzej Nasiadka, Jianli Dong, Henry M. Krause

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.

Original languageEnglish (US)
Pages (from-to)4851-4861
Number of pages11
JournalDevelopment
Volume125
Issue number23
StatePublished - 1998
Externally publishedYes

Fingerprint

Drosophila
Embryonic Development
Genes
Transcription Factors
Embryonic Structures
Phenotype
DNA
Proteins

Keywords

  • Drosophila
  • fushi tarazu
  • odd-skipped
  • Pair-rule
  • Segmentation

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Dréan, B. S. L., Nasiadka, A., Dong, J., & Krause, H. M. (1998). Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis. Development, 125(23), 4851-4861.

Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis. / Dréan, Benedicte Saulier Le; Nasiadka, Andrzej; Dong, Jianli; Krause, Henry M.

In: Development, Vol. 125, No. 23, 1998, p. 4851-4861.

Research output: Contribution to journalArticle

Dréan, BSL, Nasiadka, A, Dong, J & Krause, HM 1998, 'Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis', Development, vol. 125, no. 23, pp. 4851-4861.
Dréan, Benedicte Saulier Le ; Nasiadka, Andrzej ; Dong, Jianli ; Krause, Henry M. / Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis. In: Development. 1998 ; Vol. 125, No. 23. pp. 4851-4861.
@article{f75f6be93b89485999123db2bf5432d3,
title = "Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis",
abstract = "Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.",
keywords = "Drosophila, fushi tarazu, odd-skipped, Pair-rule, Segmentation",
author = "Dr{\'e}an, {Benedicte Saulier Le} and Andrzej Nasiadka and Jianli Dong and Krause, {Henry M.}",
year = "1998",
language = "English (US)",
volume = "125",
pages = "4851--4861",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "23",

}

TY - JOUR

T1 - Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis

AU - Dréan, Benedicte Saulier Le

AU - Nasiadka, Andrzej

AU - Dong, Jianli

AU - Krause, Henry M.

PY - 1998

Y1 - 1998

N2 - Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.

AB - Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.

KW - Drosophila

KW - fushi tarazu

KW - odd-skipped

KW - Pair-rule

KW - Segmentation

UR - http://www.scopus.com/inward/record.url?scp=20644456683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20644456683&partnerID=8YFLogxK

M3 - Article

VL - 125

SP - 4851

EP - 4861

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 23

ER -