Abstract
Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4851-4861 |
| Number of pages | 11 |
| Journal | Development |
| Volume | 125 |
| Issue number | 23 |
| State | Published - 1998 |
| Externally published | Yes |
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Keywords
- Drosophila
- fushi tarazu
- odd-skipped
- Pair-rule
- Segmentation
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis. / Dréan, Benedicte Saulier Le; Nasiadka, Andrzej; Dong, Jianli; Krause, Henry M.
In: Development, Vol. 125, No. 23, 1998, p. 4851-4861.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic changes in the functions of Odd-skipped during early Drosophila embryogenesis
AU - Dréan, Benedicte Saulier Le
AU - Nasiadka, Andrzej
AU - Dong, Jianli
AU - Krause, Henry M.
PY - 1998
Y1 - 1998
N2 - Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.
AB - Although many of the genes that pattern the segmented body plan of the Drosophila embryo are known, there remains much to learn in terms of how these genes and their products interact with one another. Like many of these gene products, the protein encoded by the pair-rule gene odd-skipped (Odd) is a DNA-binding transcription factor. Genetic experiments have suggested several candidate target genes for Odd, all of which appear to be negatively regulated. Here we use pulses of ectopic Odd expression to test the response of these and other segmentation genes. The results are complex, indicating that Odd is capable of repressing some genes wherever and whenever Odd is expressed, while the ability to repress others is temporally or spatially restricted. Moreover, one target gene fushi tarazu, is both repressed and activated by Odd, the outcome depending upon the stage of development. These results indicate that the activity of Odd is highly dependent upon the presence of cofactors and/or overriding inhibitors. Based on these results, and the segmental phenotypes generated by ectopic Odd, we suggest a number of new roles for Odd in the patterning of embryonic segments. These include gap-, pair-rule- and segment polarity-type functions.
KW - Drosophila
KW - fushi tarazu
KW - odd-skipped
KW - Pair-rule
KW - Segmentation
UR - http://www.scopus.com/inward/record.url?scp=20644456683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20644456683&partnerID=8YFLogxK
M3 - Article
C2 - 9806933
AN - SCOPUS:20644456683
VL - 125
SP - 4851
EP - 4861
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 23
ER -