Dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (SLAM, CD150)

Chanakha K. Navaratnarajah, Sompong Vongpunsawad, Numan Oezguen, Thilo Stehle, Werner Braun, Takao Hashiguchi, Katsumi Maenaka, Yusuke Yanagi, Roberto Cattaneo

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The interaction of measles virus with its receptor signaling lymphocytic activation molecule (SLAM) controls cell entry and governs tropism. We predicted potential interface areas of the measles virus attachment protein hemagglutinin to begin the investigation. We then assessed the relevance of individual amino acids located in these areas for SLAM-binding and SLAM-dependent membrane fusion, as measured by surface plasmon resonance and receptor-specific fusion assays, respectively. These studies identified one hemagglutinin protein residue, isoleucine 194, which is essential for primary binding. The crystal structure of the hemagglutinin-protein localizes Ile-194 at the interface of propeller blades 5 and 6, and our data indicate that a small aliphatic side chain of residue 194 stabilizes a protein conformation conducive to binding. In contrast, a quartet of residues previously shown to sustain SLAM-dependent fusion is not involved in binding. Instead, our data prove that after binding, this quartet of residues on propeller blade 5 conducts conformational changes that are receptor-specific. Our study sets a structure-based stage for understanding how the SLAM-elicited conformational changes travel through the H-protein ectodomain before triggering fusion protein unfolding and membrane fusion.

Original languageEnglish (US)
Pages (from-to)11763-11771
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number17
DOIs
StatePublished - Apr 25 2008

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Measles virus
Hemagglutinins
Viruses
Chemical activation
Fusion reactions
Molecules
Membrane Fusion
Proteins
Propellers
Virus Attachment
Protein Unfolding
Protein Conformation
Tropism
Surface Plasmon Resonance
Isoleucine
Membranes
Surface plasmon resonance
Conformations
Signaling Lymphocytic Activation Molecule Family
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (SLAM, CD150). / Navaratnarajah, Chanakha K.; Vongpunsawad, Sompong; Oezguen, Numan; Stehle, Thilo; Braun, Werner; Hashiguchi, Takao; Maenaka, Katsumi; Yanagi, Yusuke; Cattaneo, Roberto.

In: Journal of Biological Chemistry, Vol. 283, No. 17, 25.04.2008, p. 11763-11771.

Research output: Contribution to journalArticle

Navaratnarajah, CK, Vongpunsawad, S, Oezguen, N, Stehle, T, Braun, W, Hashiguchi, T, Maenaka, K, Yanagi, Y & Cattaneo, R 2008, 'Dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (SLAM, CD150)', Journal of Biological Chemistry, vol. 283, no. 17, pp. 11763-11771. https://doi.org/10.1074/jbc.M800896200
Navaratnarajah, Chanakha K. ; Vongpunsawad, Sompong ; Oezguen, Numan ; Stehle, Thilo ; Braun, Werner ; Hashiguchi, Takao ; Maenaka, Katsumi ; Yanagi, Yusuke ; Cattaneo, Roberto. / Dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (SLAM, CD150). In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 17. pp. 11763-11771.
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