Dynorphin A(1-8) in human placenta: Amino acid sequence determined by tandem mass spectrometry

Abdulbaki Agbas; Mahmoud S. Ahmed; William Millington; Bojana Cemerikic; Dominic M. Desiderio; Jih Lie Tseng; Chhabil Dass

doi:10.1016/0196-9781(95)00013-A

Dynorphin A(1-8) in human placenta: Amino acid sequence determined by tandem mass spectrometry

Abdulbaki Agbas, Mahmoud S. Ahmed, William Millington, Bojana Cemerikic, Dominic M. Desiderio, Jih Lie Tseng, Chhabil Dass

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Presence of the κ receptor-preferring neuropeptide dynorphin A(1-8) in human placenta has been demonstrated by mass spectrometry to establish rigorously the appropriate molecular weight and amino acid sequence. Liquid secondary ionization mass spectrometry produced the protonated molecule ion, (M + H)⁺, at m z 981 of the endogenous peptide, and tandem mass spectrometry collected the product ion spectrum that contained the appropriate amino acid sequence-determining fragment ions produced from the precursor ion (M + H)⁺. The amino acid sequence of the peptide is YGGFLRRI.

Original language	English (US)
Pages (from-to)	623-627
Number of pages	5
Journal	Peptides
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0196-9781(95)00013-A
State	Published - 1995
Externally published	Yes

Keywords

Amino acid sequence
Dynorphin A(1-8)
Human placenta

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(95)00013-A

Cite this

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title = "Dynorphin A(1-8) in human placenta: Amino acid sequence determined by tandem mass spectrometry",

abstract = "Presence of the κ receptor-preferring neuropeptide dynorphin A(1-8) in human placenta has been demonstrated by mass spectrometry to establish rigorously the appropriate molecular weight and amino acid sequence. Liquid secondary ionization mass spectrometry produced the protonated molecule ion, (M + H)+, at m z 981 of the endogenous peptide, and tandem mass spectrometry collected the product ion spectrum that contained the appropriate amino acid sequence-determining fragment ions produced from the precursor ion (M + H)+. The amino acid sequence of the peptide is YGGFLRRI.",

keywords = "Amino acid sequence, Dynorphin A(1-8), Human placenta",

author = "Abdulbaki Agbas and Ahmed, \{Mahmoud S.\} and William Millington and Bojana Cemerikic and Desiderio, \{Dominic M.\} and Tseng, \{Jih Lie\} and Chhabil Dass",

year = "1995",

doi = "10.1016/0196-9781(95)00013-A",

language = "English (US)",

volume = "16",

pages = "623--627",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Dynorphin A(1-8) in human placenta

T2 - Amino acid sequence determined by tandem mass spectrometry

AU - Agbas, Abdulbaki

AU - Ahmed, Mahmoud S.

AU - Millington, William

AU - Cemerikic, Bojana

AU - Desiderio, Dominic M.

AU - Tseng, Jih Lie

AU - Dass, Chhabil

PY - 1995

Y1 - 1995

N2 - Presence of the κ receptor-preferring neuropeptide dynorphin A(1-8) in human placenta has been demonstrated by mass spectrometry to establish rigorously the appropriate molecular weight and amino acid sequence. Liquid secondary ionization mass spectrometry produced the protonated molecule ion, (M + H)+, at m z 981 of the endogenous peptide, and tandem mass spectrometry collected the product ion spectrum that contained the appropriate amino acid sequence-determining fragment ions produced from the precursor ion (M + H)+. The amino acid sequence of the peptide is YGGFLRRI.

AB - Presence of the κ receptor-preferring neuropeptide dynorphin A(1-8) in human placenta has been demonstrated by mass spectrometry to establish rigorously the appropriate molecular weight and amino acid sequence. Liquid secondary ionization mass spectrometry produced the protonated molecule ion, (M + H)+, at m z 981 of the endogenous peptide, and tandem mass spectrometry collected the product ion spectrum that contained the appropriate amino acid sequence-determining fragment ions produced from the precursor ion (M + H)+. The amino acid sequence of the peptide is YGGFLRRI.

KW - Amino acid sequence

KW - Dynorphin A(1-8)

KW - Human placenta

UR - https://www.scopus.com/pages/publications/0029035526

UR - https://www.scopus.com/inward/citedby.url?scp=0029035526&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(95)00013-A

DO - 10.1016/0196-9781(95)00013-A

M3 - Article

C2 - 7479294

AN - SCOPUS:0029035526

SN - 0196-9781

VL - 16

SP - 623

EP - 627

JO - Peptides

JF - Peptides

IS - 4

ER -

Dynorphin A(1-8) in human placenta: Amino acid sequence determined by tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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