Abstract
Background/Aims: The transmembrane protein dystroglycan (DG) is known to anchor the cell membrane to the extracellular matrix, and is susceptible to cleavage by matrix metalloproteinases. This study tested the hypothesis that changes in DG abundance in fetal membranes (FM) occur late in gestation, with spontaneous rupture of membranes (SROM), and during labor. Methods: FM were collected from a prospective cohort consisting of four groups of patients (term labor, term unlabored, preterm labor, and preterm unlabored). FM were subjected to immunohistochemical staining using antibodies specific for α- and β-DG subunits, and staining intensity was graded by a blinded pathologist. Results: α- and β-DG staining was significantly decreased at term and after SROM (p < 0.05), but not in the presence of labor. Conclusions: Decreased DG intensity was seen in FM of patients at term and with SROM, but no change was observed with labor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 244-249 |
| Number of pages | 6 |
| Journal | Gynecologic and Obstetric Investigation |
| Volume | 79 |
| Issue number | 4 |
| DOIs | |
| State | Published - May 28 2015 |
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Keywords
- Dystroglycan
- Fetal membranes
- Spontaneous rupture of membranes
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Dystroglycan in human fetal membranes decreases in later gestation and with spontaneous membrane rupture. / Theiler, Regan N.; Snyder, Russell; Theiler, Shaleen K.
In: Gynecologic and Obstetric Investigation, Vol. 79, No. 4, 28.05.2015, p. 244-249.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dystroglycan in human fetal membranes decreases in later gestation and with spontaneous membrane rupture
AU - Theiler, Regan N.
AU - Snyder, Russell
AU - Theiler, Shaleen K.
PY - 2015/5/28
Y1 - 2015/5/28
N2 - Background/Aims: The transmembrane protein dystroglycan (DG) is known to anchor the cell membrane to the extracellular matrix, and is susceptible to cleavage by matrix metalloproteinases. This study tested the hypothesis that changes in DG abundance in fetal membranes (FM) occur late in gestation, with spontaneous rupture of membranes (SROM), and during labor. Methods: FM were collected from a prospective cohort consisting of four groups of patients (term labor, term unlabored, preterm labor, and preterm unlabored). FM were subjected to immunohistochemical staining using antibodies specific for α- and β-DG subunits, and staining intensity was graded by a blinded pathologist. Results: α- and β-DG staining was significantly decreased at term and after SROM (p < 0.05), but not in the presence of labor. Conclusions: Decreased DG intensity was seen in FM of patients at term and with SROM, but no change was observed with labor.
AB - Background/Aims: The transmembrane protein dystroglycan (DG) is known to anchor the cell membrane to the extracellular matrix, and is susceptible to cleavage by matrix metalloproteinases. This study tested the hypothesis that changes in DG abundance in fetal membranes (FM) occur late in gestation, with spontaneous rupture of membranes (SROM), and during labor. Methods: FM were collected from a prospective cohort consisting of four groups of patients (term labor, term unlabored, preterm labor, and preterm unlabored). FM were subjected to immunohistochemical staining using antibodies specific for α- and β-DG subunits, and staining intensity was graded by a blinded pathologist. Results: α- and β-DG staining was significantly decreased at term and after SROM (p < 0.05), but not in the presence of labor. Conclusions: Decreased DG intensity was seen in FM of patients at term and with SROM, but no change was observed with labor.
KW - Dystroglycan
KW - Fetal membranes
KW - Spontaneous rupture of membranes
UR - http://www.scopus.com/inward/record.url?scp=84929952783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929952783&partnerID=8YFLogxK
U2 - 10.1159/000367894
DO - 10.1159/000367894
M3 - Article
VL - 79
SP - 244
EP - 249
JO - Gynecologic and Obstetric Investigation
JF - Gynecologic and Obstetric Investigation
SN - 0378-7346
IS - 4
ER -