Abstract
The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 322-330 |
| Number of pages | 9 |
| Journal | Journal of Surgical Research |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
Cite this
Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance. / Miller, James E.; Whitman, Glenn J R; Iozzo, Renato V.; Jacobs, Danny O.; Ziegler, Moritz M.
In: Journal of Surgical Research, Vol. 39, No. 4, 1985, p. 322-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance
AU - Miller, James E.
AU - Whitman, Glenn J R
AU - Iozzo, Renato V.
AU - Jacobs, Danny O.
AU - Ziegler, Moritz M.
PY - 1985
Y1 - 1985
N2 - The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.
AB - The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.
UR - http://www.scopus.com/inward/record.url?scp=0022409243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022409243&partnerID=8YFLogxK
U2 - 10.1016/0022-4804(85)90110-6
DO - 10.1016/0022-4804(85)90110-6
M3 - Article
VL - 39
SP - 322
EP - 330
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 4
ER -