Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance

James E. Miller, Glenn J R Whitman, Renato V. Iozzo, Danny O. Jacobs, Moritz M. Ziegler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.

Original languageEnglish (US)
Pages (from-to)322-330
Number of pages9
JournalJournal of Surgical Research
Volume39
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Necrotizing Enterocolitis
Aminophylline
Protons
Early Diagnosis
Magnetic Resonance Spectroscopy
Microscopy
Superior Mesenteric Artery
Ileum
Electron Microscopy
Light
Diagnostic Imaging
Laparotomy
Theoretical Models
Necrosis
Magnetic Resonance Imaging
Mortality
Water

ASJC Scopus subject areas

  • Surgery

Cite this

Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance. / Miller, James E.; Whitman, Glenn J R; Iozzo, Renato V.; Jacobs, Danny O.; Ziegler, Moritz M.

In: Journal of Surgical Research, Vol. 39, No. 4, 1985, p. 322-330.

Research output: Contribution to journalArticle

Miller, James E. ; Whitman, Glenn J R ; Iozzo, Renato V. ; Jacobs, Danny O. ; Ziegler, Moritz M. / Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance. In: Journal of Surgical Research. 1985 ; Vol. 39, No. 4. pp. 322-330.
@article{bf7ea62bfe9c41898164b56914ba4d9c,
title = "Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance",
abstract = "The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2{\%}; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.",
author = "Miller, {James E.} and Whitman, {Glenn J R} and Iozzo, {Renato V.} and Jacobs, {Danny O.} and Ziegler, {Moritz M.}",
year = "1985",
doi = "10.1016/0022-4804(85)90110-6",
language = "English (US)",
volume = "39",
pages = "322--330",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Early diagnosis of experimental necrotizing enterocolitis using proton nuclear magnetic resonance

AU - Miller, James E.

AU - Whitman, Glenn J R

AU - Iozzo, Renato V.

AU - Jacobs, Danny O.

AU - Ziegler, Moritz M.

PY - 1985

Y1 - 1985

N2 - The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.

AB - The purpose of this study was (1) to confirm an experimental model of aminophylline-induced necrotizing enterocolitis (NEC); and (2) to determine whether nuclear magnetic resonance (NMR) imaging, based upon proton relaxation values (T1, T2), could detect NEC during its early pathogenesis. Sixty male weanling Lewis rats (avg wt = 75 g) were randomly assigned to one of three experimental groups: (A) superior mesenteric artery (SMA) occlusion (1 min) + aminophylline treatment (40 mg/kg); (B) SMA occlusion; and (C) sham midline laparotomy (control). All surviving animals were sacrificed at 48 hr postoperation and a specimen of ileum was removed for light microscopy (LM), electron microscopy (EM), and NMR analysis. Percentage water content was determined for representative specimens. Mortality occurred only in experimental group A animals (18.2%; P < 0.05), who had received aminophylline. Microscopy of ileum from sacrificed animals of this group showed changes ranging from mild cellular disruption to severe hemorrhagic necrosis. Early ultrastructural changes consistent with NEC were detectable with EM before LM. Proton relaxation results obtained with NMR showed significant prolongation of T1 (252.5 ± 4.4 msecs; P < 0.001) and T2 (69.3 ± 1.4 msecs; P < 0.025) during the first stages of NEC. NMR may indeed enable early, safe diagnostic imaging of NEC in infants receiving aminophylline, or those who are otherwise at increased risk for development of this disease.

UR - http://www.scopus.com/inward/record.url?scp=0022409243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022409243&partnerID=8YFLogxK

U2 - 10.1016/0022-4804(85)90110-6

DO - 10.1016/0022-4804(85)90110-6

M3 - Article

VL - 39

SP - 322

EP - 330

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 4

ER -