TY - JOUR
T1 - Early insights of dengue virus serotype 3 (DENV-3) re-emergence in São Paulo, Brazil
AU - Sacchetto, Lívia
AU - Bernardi, Victória
AU - Brancini, Marini L.
AU - Marques, Beatriz de C.
AU - Negri, Andreia
AU - Vasilakis, Nikos
AU - Estofolete, Cassia F.
AU - Nogueira, Maurício L.
N1 - Publisher Copyright:
© 2025 The Author(s)
PY - 2025/2
Y1 - 2025/2
N2 - Background: In dengue hyperendemic regions, the evolution of the virus is marked by frequent virus introduction/reintroduction and clade replacement events, occasionally linked to an epidemic outbreak. From 2023 onwards, an increase in the detection of DENV-3 cases has been reported in different regions of Brazil. Thus, molecular and genomic surveillance of circulating DENV strains is crucial for public health preparedness and response efforts for the disease. Objectives: This work aimed to characterize and provide preliminary insights into dengue virus serotype 3 (DENV-3) re-emergence in São Paulo state, Brazil. Study design: We conducted active arbovirus molecular surveillance on samples from patients with acute febrile illness combined with next-generation sequencing and phylogenetic analyses. Results: We detected and characterized DENV-3 circulation in São Paulo, Brazil, since late 2023. The genomes clustered within genomes recently (2022–2024) identified in Florida, the Caribbean region, and Brazil. Conclusions: Our results demonstrate the resurgence of DENV-3 in the region since 2009, raising concerns about a potential outbreak in regions with a high epidemic history.
AB - Background: In dengue hyperendemic regions, the evolution of the virus is marked by frequent virus introduction/reintroduction and clade replacement events, occasionally linked to an epidemic outbreak. From 2023 onwards, an increase in the detection of DENV-3 cases has been reported in different regions of Brazil. Thus, molecular and genomic surveillance of circulating DENV strains is crucial for public health preparedness and response efforts for the disease. Objectives: This work aimed to characterize and provide preliminary insights into dengue virus serotype 3 (DENV-3) re-emergence in São Paulo state, Brazil. Study design: We conducted active arbovirus molecular surveillance on samples from patients with acute febrile illness combined with next-generation sequencing and phylogenetic analyses. Results: We detected and characterized DENV-3 circulation in São Paulo, Brazil, since late 2023. The genomes clustered within genomes recently (2022–2024) identified in Florida, the Caribbean region, and Brazil. Conclusions: Our results demonstrate the resurgence of DENV-3 in the region since 2009, raising concerns about a potential outbreak in regions with a high epidemic history.
KW - Clade replacement
KW - Epidemic
KW - Genomic surveillance
KW - Genotype III
KW - Outbreak
KW - Re-emergence
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U2 - 10.1016/j.jcv.2025.105763
DO - 10.1016/j.jcv.2025.105763
M3 - Article
C2 - 39848015
AN - SCOPUS:85215416699
SN - 1386-6532
VL - 176
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
M1 - 105763
ER -