Early integrin binding to Arg-Gly-Asp peptide activates actin polymerization and contractile movement that stimulates outward translocation

Cheng Han Yu, Jaslyn Bee Khuan Law, Mona Suryana, Hong Yee Low, Michael Sheetz

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Integrin-mediated adhesions are critical for stem cell differentiation, cancer metastasis, and the immune response [Hynes RO (2009) Science 326:1216-1219]. However, the mechanisms of early adhesion formation remain unclear, especially the effects of lateral clustering of integrins and the role of the Src family kinases. Usingmobile Arg-Gly-Asp (RGD) peptide ligands on lipid bilayers with nano-fabricated physical barriers [Salaita K, et al. (2010) Science 327:1380-1385], we observe surprising long-range lateral movements of ligated integrins during the process of cell spreading. Initially, RGD-activated integrin clusters stimulate actin polymerization that radiates from the clusters. Myosin II contraction of actin from adjacent clusters produces contractile pairs that move toward each other against barriers. Force generated by myosin II stimulates a Src kinase-dependent lamellipodial extension and outward movement of clusters. Subsequent retraction by myosin II causes inward movement of clusters. The final cell spread area increases with the density of periodic barriers. Early integrin clustering recruits adhesion proteins, talin, paxillin, and FAK, irrespective of force generation. However, recruitment of vinculin is only observed upon contraction. Thus, we suggest that integrin activation and early clustering are independent of lateral forces. Clustering activates Src-dependent actin polymerization from clusters. Myosin contraction of clusters to lines stimulates active spreading with outward forces from actin polymerization followed by a second wave of contraction. Many of these early mechanical steps are not evident in cells spreading on immobilized matrices perhaps because of the low forces involved. These observations can provide new targets to control integrin-dependent adhesion and motility.

Original languageEnglish (US)
Pages (from-to)20585-20590
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number51
DOIs
StatePublished - Dec 20 2011
Externally publishedYes

Fingerprint

Integrins
Polymerization
Actins
Peptides
Myosin Type II
Cluster Analysis
src-Family Kinases
Talin
Paxillin
Vinculin
Architectural Accessibility
Lipid Bilayers
Myosins
Cell Differentiation
Stem Cells
Neoplasm Metastasis
Ligands
Neoplasms
Proteins

Keywords

  • Cell adhesion
  • Integrin reorganization
  • Supported membranes

ASJC Scopus subject areas

  • General

Cite this

Early integrin binding to Arg-Gly-Asp peptide activates actin polymerization and contractile movement that stimulates outward translocation. / Yu, Cheng Han; Law, Jaslyn Bee Khuan; Suryana, Mona; Low, Hong Yee; Sheetz, Michael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 51, 20.12.2011, p. 20585-20590.

Research output: Contribution to journalArticle

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