Early neural and vascular dysfunctions in diabetic rats are largely sequelae of increased sorbitol oxidation

Yasuo Ido, Jens R. Nyengaard, Kathy Chang, Ronald Tilton, Charles Kilo, Banavara L. Mylari, Peter J. Oates, Joseph R. Williamson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

These experiments were undertaken to assess the importance of cytoplasmic (c) sorbitol oxidation versus mitochondrial (m) pyruvate oxidation in mediating neural and vascular dysfunction attributable to hyperglycemia in diabetic rats. Increased oxidation of sorbitol is coupled to enzymatic reduction of free oxidized NAD +c to reduced NADHc, manifested by an increased ratio of NADH to NAD +c. Likewise, increased oxidation of pyruvate is coupled to reduction of NAD +m to NADHm, which increases the NADH/NAD +m ratio. Specific inhibitors of sorbitol production or sorbitol oxidation normalized: increased diabetic nerve NADH/NAD +c, impaired nerve-conduction velocity, and vascular dysfunction in sciatic nerve, retina, and aorta; however, they had little or no impact on increased NADH/NAD +m. These observations provide, for the first time, strong in vivo evidence for the primacy of sorbitol oxidation versus. pyruvate oxidation in mediating the metabolic imbalances, impaired nerve conduction, and vascular dysfunction evoked by diabetes. These findings are consistent with (a) the fact that oxidation of sorbitol produces "prooxidant" NADHc uncoupled from subsequent production of "antioxidant" pyruvate required for reoxidation of NADHc to NAD +c by lactate dehydrogenase, and (b) the hypothesis that neural and vascular dysfunction in early diabetes are caused primarily by increased NADHc, which fuels superoxide production by NADH-driven oxidases. Antioxid. Redox Signal.

Original languageEnglish (US)
Pages (from-to)39-51
Number of pages13
JournalAntioxidants and Redox Signaling
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Sorbitol
NAD
Blood Vessels
Rats
Oxidation
Pyruvic Acid
Neural Conduction
Medical problems
Sciatic Nerve
L-Lactate Dehydrogenase
Corrosion inhibitors
Superoxides
Hyperglycemia
Oxidation-Reduction
Aorta
Retina
Antioxidants
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Ido, Y., Nyengaard, J. R., Chang, K., Tilton, R., Kilo, C., Mylari, B. L., ... Williamson, J. R. (2010). Early neural and vascular dysfunctions in diabetic rats are largely sequelae of increased sorbitol oxidation. Antioxidants and Redox Signaling, 12(1), 39-51. https://doi.org/10.1089/ars.2009.2502

Early neural and vascular dysfunctions in diabetic rats are largely sequelae of increased sorbitol oxidation. / Ido, Yasuo; Nyengaard, Jens R.; Chang, Kathy; Tilton, Ronald; Kilo, Charles; Mylari, Banavara L.; Oates, Peter J.; Williamson, Joseph R.

In: Antioxidants and Redox Signaling, Vol. 12, No. 1, 01.01.2010, p. 39-51.

Research output: Contribution to journalArticle

Ido, Y, Nyengaard, JR, Chang, K, Tilton, R, Kilo, C, Mylari, BL, Oates, PJ & Williamson, JR 2010, 'Early neural and vascular dysfunctions in diabetic rats are largely sequelae of increased sorbitol oxidation', Antioxidants and Redox Signaling, vol. 12, no. 1, pp. 39-51. https://doi.org/10.1089/ars.2009.2502
Ido, Yasuo ; Nyengaard, Jens R. ; Chang, Kathy ; Tilton, Ronald ; Kilo, Charles ; Mylari, Banavara L. ; Oates, Peter J. ; Williamson, Joseph R. / Early neural and vascular dysfunctions in diabetic rats are largely sequelae of increased sorbitol oxidation. In: Antioxidants and Redox Signaling. 2010 ; Vol. 12, No. 1. pp. 39-51.
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