Early production of type I interferon during West Nile virus infection: Role for lymphoid tissues in IRF3-independent interferon production

Nigel Bourne, Frank Scholle, Maria Carlan Silva, Shannan Rossi, Nathan Dewsbury, Barbara Judy, Juliana B. De Aguiar, Megan A. Leon, D. Mark Estes, Rafik Fayzulin, Peter W. Mason

Research output: Contribution to journalArticle

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Abstract

Infection of cells with flaviviruses in vitro is reduced by pretreatment with small amounts of type I interferon (IFN-α/β). Similarly, pretreatment of animals with IFN and experiments using mice defective in IFN signaling have indicated a role for IFN in controlling flavivirus disease in vivo. These data, along with findings that flavivirus-infected cells block IFN signaling, suggest that flavivirus infection can trigger an IFN response. To investigate IFN gene induction by the very first cells infected during in vivo infection with the flavivirus West Nile virus (WNV), we infected mice with high-titer preparations of WNV virus-like particles (VLPs), which initiate viral genome replication in cells but fail to spread. These studies demonstrated a brisk production of IFN in vivo, with peak levels of over 1,000 units/ml detected in sera between 8 and 24 h after inoculation by either the intraperitoneal or footpad route. The IFN response was dependent on genome replication, and WNV genomes and WNV antigen-positive cells were readily detected in the popliteal lymph nodes (pLN) of VLP-inoculated mice. High levels of IFN mRNA transcripts and functional IFN were also produced in VLP-inoculated IFN regulatory factor 3 null (IRF3_/_) mice, indicating that IFN production was independent of the IRF3 pathways to IFN gene transcription, consistent with the IFN type produced (predominantly α).

Original languageEnglish (US)
Pages (from-to)9100-9108
Number of pages9
JournalJournal of Virology
Volume81
Issue number17
DOIs
StatePublished - Sep 2007

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West Nile virus
Interferon Type I
Lymphoid Tissue
Virus Diseases
interferons
Flavivirus
Interferons
virus-like particles
Flavivirus Infections
Virion
infection
mice
genome
cells
pretreatment
Interferon Regulatory Factor-3
Genome
Flaviviridae
Viral Genome
gene induction

ASJC Scopus subject areas

  • Immunology

Cite this

Early production of type I interferon during West Nile virus infection : Role for lymphoid tissues in IRF3-independent interferon production. / Bourne, Nigel; Scholle, Frank; Silva, Maria Carlan; Rossi, Shannan; Dewsbury, Nathan; Judy, Barbara; De Aguiar, Juliana B.; Leon, Megan A.; Estes, D. Mark; Fayzulin, Rafik; Mason, Peter W.

In: Journal of Virology, Vol. 81, No. 17, 09.2007, p. 9100-9108.

Research output: Contribution to journalArticle

Bourne, N, Scholle, F, Silva, MC, Rossi, S, Dewsbury, N, Judy, B, De Aguiar, JB, Leon, MA, Estes, DM, Fayzulin, R & Mason, PW 2007, 'Early production of type I interferon during West Nile virus infection: Role for lymphoid tissues in IRF3-independent interferon production', Journal of Virology, vol. 81, no. 17, pp. 9100-9108. https://doi.org/10.1128/JVI.00316-07
Bourne, Nigel ; Scholle, Frank ; Silva, Maria Carlan ; Rossi, Shannan ; Dewsbury, Nathan ; Judy, Barbara ; De Aguiar, Juliana B. ; Leon, Megan A. ; Estes, D. Mark ; Fayzulin, Rafik ; Mason, Peter W. / Early production of type I interferon during West Nile virus infection : Role for lymphoid tissues in IRF3-independent interferon production. In: Journal of Virology. 2007 ; Vol. 81, No. 17. pp. 9100-9108.
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