Abstract
Screening of monoclonal antibodies against ebolaviruses requires small-animal models. Wild-type mice require adaptation of ebolaviruses, whereas immunodeficient mice are still resistant to nonadapted Bundibugyo ebolavirus. Swapping of Ebola virus glycoprotein with that from Bundibugyo virus resulted in a replication-competent chimeric virus, which caused 100% lethal infection in STAT1 knockout mice. Monoclonal antibody BDBV223 isolated from a human survivor of Bundibugyo virus infection protected mice from challenge with the chimeric virus. These data demonstrate the suitability of the approach for in vivo screening of antibodies and suggest the greater contribution of internal Ebola proteins in pathogenesis compared to Bundibugyo virus proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | S418-S422 |
| Journal | The Journal of infectious diseases |
| Volume | 218 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 22 2018 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
Cite this
Ebolavirus Chimerization for the Development of a Mouse Model for Screening of Bundibugyo-Specific Antibodies. / Ilinykh, Philipp A.; Graber, Jessica; Kuzmina, Natalia A.; Huang, Kai; Ksiazek, Thomas; Crowe, James E.; Bukreyev, Alexander.
In: The Journal of infectious diseases, Vol. 218, No. 5, 22.11.2018, p. S418-S422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ebolavirus Chimerization for the Development of a Mouse Model for Screening of Bundibugyo-Specific Antibodies
AU - Ilinykh, Philipp A.
AU - Graber, Jessica
AU - Kuzmina, Natalia A.
AU - Huang, Kai
AU - Ksiazek, Thomas
AU - Crowe, James E.
AU - Bukreyev, Alexander
PY - 2018/11/22
Y1 - 2018/11/22
N2 - Screening of monoclonal antibodies against ebolaviruses requires small-animal models. Wild-type mice require adaptation of ebolaviruses, whereas immunodeficient mice are still resistant to nonadapted Bundibugyo ebolavirus. Swapping of Ebola virus glycoprotein with that from Bundibugyo virus resulted in a replication-competent chimeric virus, which caused 100% lethal infection in STAT1 knockout mice. Monoclonal antibody BDBV223 isolated from a human survivor of Bundibugyo virus infection protected mice from challenge with the chimeric virus. These data demonstrate the suitability of the approach for in vivo screening of antibodies and suggest the greater contribution of internal Ebola proteins in pathogenesis compared to Bundibugyo virus proteins.
AB - Screening of monoclonal antibodies against ebolaviruses requires small-animal models. Wild-type mice require adaptation of ebolaviruses, whereas immunodeficient mice are still resistant to nonadapted Bundibugyo ebolavirus. Swapping of Ebola virus glycoprotein with that from Bundibugyo virus resulted in a replication-competent chimeric virus, which caused 100% lethal infection in STAT1 knockout mice. Monoclonal antibody BDBV223 isolated from a human survivor of Bundibugyo virus infection protected mice from challenge with the chimeric virus. These data demonstrate the suitability of the approach for in vivo screening of antibodies and suggest the greater contribution of internal Ebola proteins in pathogenesis compared to Bundibugyo virus proteins.
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UR - http://www.scopus.com/inward/citedby.url?scp=85057162929&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiy423
DO - 10.1093/infdis/jiy423
M3 - Article
C2 - 30060231
AN - SCOPUS:85057162929
VL - 218
SP - S418-S422
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -