@article{ec3b4ea317124eefaee0ea1b731c1e64,
title = "Ebolavirus Chimerization for the Development of a Mouse Model for Screening of Bundibugyo-Specific Antibodies",
abstract = "Screening of monoclonal antibodies against ebolaviruses requires small-animal models. Wild-type mice require adaptation of ebolaviruses, whereas immunodeficient mice are still resistant to nonadapted Bundibugyo ebolavirus. Swapping of Ebola virus glycoprotein with that from Bundibugyo virus resulted in a replication-competent chimeric virus, which caused 100% lethal infection in STAT1 knockout mice. Monoclonal antibody BDBV223 isolated from a human survivor of Bundibugyo virus infection protected mice from challenge with the chimeric virus. These data demonstrate the suitability of the approach for in vivo screening of antibodies and suggest the greater contribution of internal Ebola proteins in pathogenesis compared to Bundibugyo virus proteins.",
keywords = "Bundibugyo virus, Ebola virus, glycoprotein, monoclonal antibodies, mouse model",
author = "Ilinykh, {Philipp A.} and Jessica Graber and Kuzmina, {Natalia A.} and Kai Huang and Ksiazek, {Thomas G.} and Crowe, {James E.} and Alexander Bukreyev",
note = "Funding Information: clone, and Drs Y. Kawaoka (University of Wisconsin) and H. Feldmann (National Institutes of Health) for providing the EBOV NP, VP35, L, VP30, and T7 polymerase plasmids. We thank the UTMB Animal Resource Center veterinary staff for their excellent technical support of mouse experiments in ABSL-4. This study was supported by the Defense Threat Reduction Agency (grant number HDTRA1-13-1-0034 to J. E. C. and A. B.) and the National Institute of Allergy and Infectious Diseases (grant number U19 AI109711 to J. E. C. and A. B.). Publisher Copyright: {\textcopyright} 2018. This article contains public sector information licensed under the Open Government Licence v3.0.",
year = "2018",
month = nov,
day = "22",
doi = "10.1093/infdis/jiy423",
language = "English (US)",
volume = "218",
pages = "S418--S422",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
}