Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia

Translated title of the contribution: Ecoepidemiology of rickettsial infection in rodents, ectoparasites and humans in northeastern Antioquia, Colombia

Juan Carlos Quintero, Andrés Felipe Londoño, Francisco J. Díaz, Piedad Agudelo-Flórez, Margarita Arboleda, Juan David Rodas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Introduction: Rickettsia spp. are tick, flea or lice-borne pathogenic bacterium, usually carried by rodents. In the last decade three outbreaks of rickettsial disease including fatalities, occurred in the provinces of Antioquia and Córdoba in northwestern Colombia. Objective: The purpose of this study was to perform an ecological and epidemiological description of the Rickettsia spp infection in the recently affected region of Colombia. Materials and methods: Samples were obtained from 354 rodents and their parasites captured in the municipalities of Apartadó, Turbo and Necoclí. Likewise, 220 human sera were also collected, for detection of infection by Rickettsia spp. Results: Indirect immunofluorescence assay (IFA) revealed that 130 (43%) of the rodents and 53 (24%) of the humans produced antibodies to Rickettsia spp. Additionally, rickettsial DNA was amplified by PCR from 23 (6.8%) rodent liver samples using primers directed to the genus specific gltA gene. While gltA sequences from rodent samples exhibited a 98.7% similitude with R. prowazekii, a sequence amplified from larvae of Amblyomma sp exhibited identities of >99% similarity with R. tamurae. Conclusion: These results demonstrate the presence of rickettsia in rodents, ectoparasites and humans throughout the municipalities studied.

Original languageSpanish
Pages (from-to)38-51
Number of pages14
JournalBiomedica
Volume33
Issue numberSUPPL.1
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

Colombia
Rodentia
Rickettsia
Rickettsia Infections
Infection
Phthiraptera
Siphonaptera
Ticks
Indirect Fluorescent Antibody Technique
Liver
Disease Outbreaks
Larva
Assays
Bacteria
Parasites
Genes
Polymerase Chain Reaction
Antibodies
DNA
Serum

Keywords

  • Colombia
  • Diagnosis
  • Disease vectors
  • Rickettsia
  • Rocky Mountain spotted fever
  • Typhoid fever

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Quintero, J. C., Londoño, A. F., Díaz, F. J., Agudelo-Flórez, P., Arboleda, M., & Rodas, J. D. (2013). Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia. Biomedica, 33(SUPPL.1), 38-51. https://doi.org/10.7705/biomedica.v33i0.735

Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia. / Quintero, Juan Carlos; Londoño, Andrés Felipe; Díaz, Francisco J.; Agudelo-Flórez, Piedad; Arboleda, Margarita; Rodas, Juan David.

In: Biomedica, Vol. 33, No. SUPPL.1, 01.09.2013, p. 38-51.

Research output: Contribution to journalArticle

Quintero, JC, Londoño, AF, Díaz, FJ, Agudelo-Flórez, P, Arboleda, M & Rodas, JD 2013, 'Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia', Biomedica, vol. 33, no. SUPPL.1, pp. 38-51. https://doi.org/10.7705/biomedica.v33i0.735
Quintero, Juan Carlos ; Londoño, Andrés Felipe ; Díaz, Francisco J. ; Agudelo-Flórez, Piedad ; Arboleda, Margarita ; Rodas, Juan David. / Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia. In: Biomedica. 2013 ; Vol. 33, No. SUPPL.1. pp. 38-51.
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abstract = "Introduction: Rickettsia spp. are tick, flea or lice-borne pathogenic bacterium, usually carried by rodents. In the last decade three outbreaks of rickettsial disease including fatalities, occurred in the provinces of Antioquia and C{\'o}rdoba in northwestern Colombia. Objective: The purpose of this study was to perform an ecological and epidemiological description of the Rickettsia spp infection in the recently affected region of Colombia. Materials and methods: Samples were obtained from 354 rodents and their parasites captured in the municipalities of Apartad{\'o}, Turbo and Necocl{\'i}. Likewise, 220 human sera were also collected, for detection of infection by Rickettsia spp. Results: Indirect immunofluorescence assay (IFA) revealed that 130 (43{\%}) of the rodents and 53 (24{\%}) of the humans produced antibodies to Rickettsia spp. Additionally, rickettsial DNA was amplified by PCR from 23 (6.8{\%}) rodent liver samples using primers directed to the genus specific gltA gene. While gltA sequences from rodent samples exhibited a 98.7{\%} similitude with R. prowazekii, a sequence amplified from larvae of Amblyomma sp exhibited identities of >99{\%} similarity with R. tamurae. Conclusion: These results demonstrate the presence of rickettsia in rodents, ectoparasites and humans throughout the municipalities studied.",
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