Ectopic expression of cyclin D3 corrects differentiation of DM1 myoblasts through activation of RNA CUG-binding protein, CUGBP1

Elizabeth Salisbury, Keiko Sakai, Benedikt Schoser, Claudia Huichalaf, Christiane Schneider-Gold, Heather Nguyen, Gou Li Wang, Jeffrey H. Albrecht, Lubov T. Timchenko

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Differentiation of myocytes is impaired in patients with myotonic dystrophy type 1, DM1. CUG repeat binding protein, CUGBP1, is a key regulator of translation of proteins that are involved in muscle development and differentiation. In this paper, we present evidence that RNA-binding activity of CUGBP1 and its interactions with initiation translation complex eIF2 are differentially regulated during myogenesis by specific phosphorylation and that this regulation is altered in DM1. In normal myoblasts, Akt kinase phosphorylates CUGBP1 at Ser28 and increases interactions of CUGBP1 with cyclin D1 mRNA. During differentiation, CUGBP1 is phosphorylated by cyclinD3-cdk4/6 at Ser302, which increases CUGBP1 binding with p21 and C/EBPβ mRNAs. While cyclin D3 and cdk4 are elevated in normal myotubes; DM1 differentiating cells do not increase these proteins. In normal myotubes, CUGBP1 interacts with cyclin D3/cdk4/6 and eIF2; however, interactions of CUGBP1 with eIF2 are reduced in DM1 differentiating cells and correlate with impaired muscle differentiation in DM1. Ectopic expression of cyclin D3 in DM1 cells increases the CUGBP1-eIF2 complex, corrects expression of differentiation markers, myogenin and desmin, and enhances fusion of DM1 myoblasts. Thus, normalization of cyclin D3 might be a therapeutic approach to correct differentiation of skeletal muscle in DM1 patients.

Original languageEnglish (US)
Pages (from-to)2266-2278
Number of pages13
JournalExperimental Cell Research
Volume314
Issue number11-12
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Cyclin D3
RNA-Binding Proteins
Myoblasts
Muscle Development
Skeletal Muscle Fibers
Myogenin
Myotonic Dystrophy
Messenger RNA
Desmin
Cyclin D1
Differentiation Antigens
Protein Biosynthesis
Muscle Cells
Carrier Proteins
Skeletal Muscle
Phosphotransferases
Phosphorylation
RNA
Muscles
Ectopic Gene Expression

Keywords

  • CUG repeats
  • CUGBP1-eIF2 complex
  • Cyclin D3
  • Differentiation
  • Myotonic dystrophy 1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ectopic expression of cyclin D3 corrects differentiation of DM1 myoblasts through activation of RNA CUG-binding protein, CUGBP1. / Salisbury, Elizabeth; Sakai, Keiko; Schoser, Benedikt; Huichalaf, Claudia; Schneider-Gold, Christiane; Nguyen, Heather; Wang, Gou Li; Albrecht, Jeffrey H.; Timchenko, Lubov T.

In: Experimental Cell Research, Vol. 314, No. 11-12, 2008, p. 2266-2278.

Research output: Contribution to journalArticle

Salisbury, E, Sakai, K, Schoser, B, Huichalaf, C, Schneider-Gold, C, Nguyen, H, Wang, GL, Albrecht, JH & Timchenko, LT 2008, 'Ectopic expression of cyclin D3 corrects differentiation of DM1 myoblasts through activation of RNA CUG-binding protein, CUGBP1', Experimental Cell Research, vol. 314, no. 11-12, pp. 2266-2278. https://doi.org/10.1016/j.yexcr.2008.04.018
Salisbury, Elizabeth ; Sakai, Keiko ; Schoser, Benedikt ; Huichalaf, Claudia ; Schneider-Gold, Christiane ; Nguyen, Heather ; Wang, Gou Li ; Albrecht, Jeffrey H. ; Timchenko, Lubov T. / Ectopic expression of cyclin D3 corrects differentiation of DM1 myoblasts through activation of RNA CUG-binding protein, CUGBP1. In: Experimental Cell Research. 2008 ; Vol. 314, No. 11-12. pp. 2266-2278.
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AU - Nguyen, Heather

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