Effect of 3,5-di-t-butyl-4-hydroxytoluene (BHT) on glutathione-linked detoxification mechanisms of rat ocular lens

Shivendra V. Singh, Satish Srivastava, Yogesh C. Awasthi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

When rats were orally administered a daily dose of 300 mg kg-1 body weight of 3,5-di-t-butyl-4-hydroxytoluene (BHT) for 4 days, about 90% increase over basal level in total glutathione (GSH) S-transferase activity towards 1-chloro-2,4-dinitrobenzene (CDNB) was observed in ocular lens. GSH S-transferase activity in the ocular lens was also increased towards other substrates such as p-nitrobenzyl chloride and ethacrynic acid. In the rat lens, two isoenzymes of GSH S-transferase (pI 8·0 and 6·1) are present, and both of these isoenzymes are induced by BHT treatment. The quantification of GSH S-transferase protein in the control and the BHT-treated rat lenses indicates that the increase in GSH S-transferase activity in the ocular lens is due to the increased enzyme protein and not due to the activation of the enzyme. A significant increase in glutathione (acid soluble thiol) levels and glutathione reductase activity was also observed in the lenses of rats treated with BHT. Glutathione peroxidase activity and the enzymes of mercapturic acid pathway except GSH S-transferase remained unaltered by the BHT treatment.

Original languageEnglish (US)
Pages (from-to)405-413
Number of pages9
JournalExperimental Eye Research
Volume41
Issue number3
DOIs
StatePublished - 1985

Fingerprint

Butylated Hydroxytoluene
Crystalline Lens
Transferases
Glutathione
Lenses
Isoenzymes
Ethacrynic Acid
Dinitrochlorobenzene
Enzyme Activation
Glutathione Reductase
Protein S
Acetylcysteine
Enzymes
Glutathione Peroxidase
Glutathione Transferase
Sulfhydryl Compounds
Body Weight
Acids
Proteins

Keywords

  • antioxidants
  • glutathione
  • glutathione peroxidase
  • glutathione reductase
  • glutathione S-transferase
  • t-butylated hydroxytoluene

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Effect of 3,5-di-t-butyl-4-hydroxytoluene (BHT) on glutathione-linked detoxification mechanisms of rat ocular lens. / Singh, Shivendra V.; Srivastava, Satish; Awasthi, Yogesh C.

In: Experimental Eye Research, Vol. 41, No. 3, 1985, p. 405-413.

Research output: Contribution to journalArticle

Singh, Shivendra V. ; Srivastava, Satish ; Awasthi, Yogesh C. / Effect of 3,5-di-t-butyl-4-hydroxytoluene (BHT) on glutathione-linked detoxification mechanisms of rat ocular lens. In: Experimental Eye Research. 1985 ; Vol. 41, No. 3. pp. 405-413.
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