Effect of a bradykinin antagonist on the local inflammatory response following thermal injury

F. E. Nwariaku, P. J. Sikes, E. Lightfoot, William Mileski, C. Baxter

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Partial and full thickness burns with intervening zones of stasis were created on the backs of New Zealand White rabbits (n = 23). Either saline or the bradykinin receptor antagonist, NPC 17731, was administered. Skin blood flow was measured hourly using a laser Doppler blood flowmeter. After 4h skin samples were harvested for assessment of tissue oedema (wet/dry weights) and leucocyte accumulation (immunohistochemistry). Statistical analysis was performed using Analysis of Variance (ANOVA) and Mann-Whitney U test with a level of significance at P < 0.005. It was found that blood flow was decreased postburn in all groups. Bradykinin antagonist resulted in increased blood flow in partial thickness burns and zones of stasis compared to saline-treated animals (P < 0.05). Pretreatment with bradykinin antagonist showed reduced tissue oedema in leucocyte burns (P < 0.05). No significant difference was observed in leucocyte accumulation between both groups. These data suggest a role for bradykinin in the pathogenesis of postburn microvascular changes which is independent of leucocyte-mediated injury.

Original languageEnglish (US)
Pages (from-to)324-327
Number of pages4
JournalBurns
Volume22
Issue number4
DOIs
StatePublished - Jun 1996
Externally publishedYes

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Leukocytes
Hot Temperature
Burns
Wounds and Injuries
Edema
Flowmeters
Skin
Bradykinin
Nonparametric Statistics
Analysis of Variance
Lasers
Immunohistochemistry
Rabbits
Weights and Measures
Bradykinin Receptor Antagonists

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Effect of a bradykinin antagonist on the local inflammatory response following thermal injury. / Nwariaku, F. E.; Sikes, P. J.; Lightfoot, E.; Mileski, William; Baxter, C.

In: Burns, Vol. 22, No. 4, 06.1996, p. 324-327.

Research output: Contribution to journalArticle

Nwariaku, F. E. ; Sikes, P. J. ; Lightfoot, E. ; Mileski, William ; Baxter, C. / Effect of a bradykinin antagonist on the local inflammatory response following thermal injury. In: Burns. 1996 ; Vol. 22, No. 4. pp. 324-327.
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