Effect of a combination therapy between IL-12 and soluble IL-4 receptor (sIL-4R) on Candida albicans and herpes simplex virus type 1 infections in thermally injured mice

Makiko Kobayashi, Hitoshi Takahashi, David Herndon, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effectiveness of a combination using IL-12 and soluble IL-4 receptor (sIL-4R) to treat severe infections of herpes simplex virus type 1 (HSV-1) and Candida albicans in thermally injured mice was investigated. Although sIL-4R decreased burn-associated type 2 T-cell responses, the effect of sIL-4R was minimal on the morbidity and mortality of thermally injured mice exposed to 250 times LD50 of HSV-1 or 10 times LD50 of C. albicans. Compared with 100% mortality in control mice, mortality for HSV-1 and C. albicans was 40 and 20%, respectively, in thermally injured mice that received IL-12 and sIL-4R in combination. After stimulation with anti-CD3 monoclonal antibody, splenic T cells from thermally injured mice exposed to large amounts of HSV-1 or C. albicans did not produce gamma interferon (IFN-γ) into their culture fluids. However, IFN-γ was produced by splenic T cells from thermally injured and infected mice treated with IL-12 and sIL-4R in combination. These results suggest that therapeutic treatment with a combination of IL-12 and sIL-4R may be effective by inducing type 1 T-cell responses in thermally injured mice exposed to large amounts of HSV-1 or C. albicans.

Original languageEnglish (US)
Pages (from-to)886-894
Number of pages9
JournalCanadian Journal of Microbiology
Volume48
Issue number10
DOIs
StatePublished - Oct 1 2002

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Interleukin-4 Receptors
Candida
Human Herpesvirus 1
Virus Diseases
Interleukin-12
Candida albicans
Viruses
T-cells
T-Lymphocytes
Lethal Dose 50
Therapeutics
Mortality
Interferons
Interferon-gamma
Burns
Monoclonal Antibodies
Fluids
Morbidity
Infection

Keywords

  • Burn
  • Candida albicans
  • Herpesvirus
  • IL-12
  • Soluble IL-4 receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Effect of a combination therapy between IL-12 and soluble IL-4 receptor (sIL-4R) on Candida albicans and herpes simplex virus type 1 infections in thermally injured mice. / Kobayashi, Makiko; Takahashi, Hitoshi; Herndon, David; Pollard, Richard B.; Suzuki, Fujio.

In: Canadian Journal of Microbiology, Vol. 48, No. 10, 01.10.2002, p. 886-894.

Research output: Contribution to journalArticle

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abstract = "The effectiveness of a combination using IL-12 and soluble IL-4 receptor (sIL-4R) to treat severe infections of herpes simplex virus type 1 (HSV-1) and Candida albicans in thermally injured mice was investigated. Although sIL-4R decreased burn-associated type 2 T-cell responses, the effect of sIL-4R was minimal on the morbidity and mortality of thermally injured mice exposed to 250 times LD50 of HSV-1 or 10 times LD50 of C. albicans. Compared with 100{\%} mortality in control mice, mortality for HSV-1 and C. albicans was 40 and 20{\%}, respectively, in thermally injured mice that received IL-12 and sIL-4R in combination. After stimulation with anti-CD3 monoclonal antibody, splenic T cells from thermally injured mice exposed to large amounts of HSV-1 or C. albicans did not produce gamma interferon (IFN-γ) into their culture fluids. However, IFN-γ was produced by splenic T cells from thermally injured and infected mice treated with IL-12 and sIL-4R in combination. These results suggest that therapeutic treatment with a combination of IL-12 and sIL-4R may be effective by inducing type 1 T-cell responses in thermally injured mice exposed to large amounts of HSV-1 or C. albicans.",
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