Effect of a previous pregnancy on vascular function in endothelial nitric oxide synthase 3 knockout mice

Labib M. Ghulmiyyah, Esther Tamayo, Shannon Clark, Gary Hankins, Garland D. Anderson, George Saade, Monica Longo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: Nitric oxide deficiency has been implicated in adverse pregnancy outcomes. Mice that lack endothelial nitric oxide synthase (NOS3) have abnormal in vitro vascular reactivity. Our objective was to assess the effect of a previous pregnancy on the abnormal vascular function of NOS3 knockout mice. Study Design: Carotid arteries from pregnant NOS3 knockout (NOS3-/-KO) and wild-type control mice (NOS3+/+WT) from first and second pregnancy were obtained for in vitro vascular reactivity studies. Vascular responses to cumulative concentrations of the vasoconstrictors phenylephrine, serotonin, and thromboxane and the vasorelaxants acetylcholine, sodium nitroprusside, and isoproterenol were determined. Results: In the first pregnancy, contractile responses were exaggerated in the knockout animals, compared with the wild-type animals. However, the second pregnancy in knockout animals was associated with normalization of responses to phenylephrine and serotonin and increased responses to the endothelium-independent relaxants. Conclusion: The vascular function of NOS3 knockout mice improves with subsequent pregnancy becoming comparable to wild-type animals.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume197
Issue number3
DOIs
StatePublished - Sep 2007

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Nitric Oxide Synthase Type III
Knockout Mice
Blood Vessels
Pregnancy
Wild Animals
Phenylephrine
Serotonin
Thromboxanes
Nitroprusside
Vasoconstrictor Agents
Pregnancy Outcome
Vasodilator Agents
Isoproterenol
Carotid Arteries
Acetylcholine
Endothelium
Nitric Oxide
In Vitro Techniques

Keywords

  • fetal programming
  • subsequent pregnancy
  • vascular reactivity

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effect of a previous pregnancy on vascular function in endothelial nitric oxide synthase 3 knockout mice. / Ghulmiyyah, Labib M.; Tamayo, Esther; Clark, Shannon; Hankins, Gary; Anderson, Garland D.; Saade, George; Longo, Monica.

In: American Journal of Obstetrics and Gynecology, Vol. 197, No. 3, 09.2007.

Research output: Contribution to journalArticle

Ghulmiyyah, Labib M. ; Tamayo, Esther ; Clark, Shannon ; Hankins, Gary ; Anderson, Garland D. ; Saade, George ; Longo, Monica. / Effect of a previous pregnancy on vascular function in endothelial nitric oxide synthase 3 knockout mice. In: American Journal of Obstetrics and Gynecology. 2007 ; Vol. 197, No. 3.
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