Abstract
Effect of Kanzo-bushi-to (TJS-038), one of the Japanese Kampo medicines originating from traditional Chinese herbal medicine, on the activity of a clone of burn-associated CD8+ suppressor T cells (T6S cells) was investigated. The suppressor cell activity of T6S cells in the mixed lymphocyte-tumor cell reaction (MLTR) was not changed when various concentrations of TJS-038 were added to the MLTR. The suppressor cell activity of T6S cells was greatly inhibited by CD3+ CD4+ CD28+ TCRα/β+ Vicia villosa lectin-adherent cells (TJS cells) purified from spleen cells of mice treated with TJS-038 at an oral dose of 50 mg/kg. The suppressor cell activity of T6S cells was not demonstrated in the MLTR assay when anti-IL-4 monoclonal antibody was added. Also the production of IL-4 from T6S cells was not detected, when they were co-cultured with TJS cells. These results suggest that TJS-038 stimulates the generation of contrasuppressor T cells (TJS cells) that inhibit the suppressor cell activity of T6S cells and the production of IL-4 from T6S cells.
Original language | English (US) |
---|---|
Pages (from-to) | 13-20 |
Number of pages | 8 |
Journal | Immunology Letters |
Volume | 40 |
Issue number | 1 |
DOIs | |
State | Published - 1994 |
Fingerprint
Keywords
- Contrasuppressor T cell
- Interleukin-4
- Mixed lymphocyte-tumor cell reaction
- Suppressor T cell clone
- Thermal injury
- Traditional Chinese herbal medicine
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Effect of a traditional Chinese herbal medicine, Kanzo-bushi-to, on the production of interleukin-4 from a clone of burn-associated CD8+ suppressor T cells. / Kobayashi, Makiko; Utsunomiya, Tokuichiro; Herndon, David; Pollard, Richard B.; Suzuki, Fujio.
In: Immunology Letters, Vol. 40, No. 1, 1994, p. 13-20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of a traditional Chinese herbal medicine, Kanzo-bushi-to, on the production of interleukin-4 from a clone of burn-associated CD8+ suppressor T cells
AU - Kobayashi, Makiko
AU - Utsunomiya, Tokuichiro
AU - Herndon, David
AU - Pollard, Richard B.
AU - Suzuki, Fujio
PY - 1994
Y1 - 1994
N2 - Effect of Kanzo-bushi-to (TJS-038), one of the Japanese Kampo medicines originating from traditional Chinese herbal medicine, on the activity of a clone of burn-associated CD8+ suppressor T cells (T6S cells) was investigated. The suppressor cell activity of T6S cells in the mixed lymphocyte-tumor cell reaction (MLTR) was not changed when various concentrations of TJS-038 were added to the MLTR. The suppressor cell activity of T6S cells was greatly inhibited by CD3+ CD4+ CD28+ TCRα/β+ Vicia villosa lectin-adherent cells (TJS cells) purified from spleen cells of mice treated with TJS-038 at an oral dose of 50 mg/kg. The suppressor cell activity of T6S cells was not demonstrated in the MLTR assay when anti-IL-4 monoclonal antibody was added. Also the production of IL-4 from T6S cells was not detected, when they were co-cultured with TJS cells. These results suggest that TJS-038 stimulates the generation of contrasuppressor T cells (TJS cells) that inhibit the suppressor cell activity of T6S cells and the production of IL-4 from T6S cells.
AB - Effect of Kanzo-bushi-to (TJS-038), one of the Japanese Kampo medicines originating from traditional Chinese herbal medicine, on the activity of a clone of burn-associated CD8+ suppressor T cells (T6S cells) was investigated. The suppressor cell activity of T6S cells in the mixed lymphocyte-tumor cell reaction (MLTR) was not changed when various concentrations of TJS-038 were added to the MLTR. The suppressor cell activity of T6S cells was greatly inhibited by CD3+ CD4+ CD28+ TCRα/β+ Vicia villosa lectin-adherent cells (TJS cells) purified from spleen cells of mice treated with TJS-038 at an oral dose of 50 mg/kg. The suppressor cell activity of T6S cells was not demonstrated in the MLTR assay when anti-IL-4 monoclonal antibody was added. Also the production of IL-4 from T6S cells was not detected, when they were co-cultured with TJS cells. These results suggest that TJS-038 stimulates the generation of contrasuppressor T cells (TJS cells) that inhibit the suppressor cell activity of T6S cells and the production of IL-4 from T6S cells.
KW - Contrasuppressor T cell
KW - Interleukin-4
KW - Mixed lymphocyte-tumor cell reaction
KW - Suppressor T cell clone
KW - Thermal injury
KW - Traditional Chinese herbal medicine
UR - http://www.scopus.com/inward/record.url?scp=0028132663&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028132663&partnerID=8YFLogxK
U2 - 10.1016/0165-2478(94)90199-6
DO - 10.1016/0165-2478(94)90199-6
M3 - Article
C2 - 7927508
AN - SCOPUS:0028132663
VL - 40
SP - 13
EP - 20
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 1
ER -