Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women

Melissa M. Markofski, Jared M. Dickinson, Micah J. Drummond, Christopher Fry, Satoshi Fujita, David M. Gundermann, Erin L. Glynn, Kristofer Jennings, Douglas Paddon-Jones, Paul T. Reidy, Melinda Sheffield-Moore, Kyle L. Timmerman, Blake Rasmussen, Elena Volpi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The rate of muscle loss with aging is higher in men than women. However, women have smaller muscles throughout the adult life. Protein content is a major determinant of skeletal muscle size. This study was designed to determine if age and sex differentially impact basal muscle protein synthesis and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling. We performed a secondary data analysis on a cohort of 215 healthy, non-obese (BMI<30kg·m-2) young (18-40y; 74 men, 52 women) and older (60-87y; 57 men, 32 women) adults. The database contained information on physical characteristics, basal muscle protein fractional synthetic rate (FSR; n=215; stable isotope methodology) and mTORC1 signaling (n=125, Western blotting). FSR and mTORC1 signaling were measured at rest and after an overnight fast. mTORC1 and S6K1 phosphorylation were higher (p<0.05) in older subjects with no sex differences. However, there were no age or sex differences or interaction for muscle FSR (p>0.05). Body mass index, fat free mass, or body fat was not a significant covariate and did not influence the results. We conclude that age and sex do not influence basal muscle protein synthesis. However, basal mTORC1 hyperphosphorylation in the elderly may contribute to insulin resistance and the age-related anabolic resistance of skeletal muscle protein metabolism to nutrition and exercise.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalExperimental Gerontology
Volume65
DOIs
StatePublished - May 1 2015

Fingerprint

Muscle Proteins
Muscle
Skeletal Muscle
Fats
Muscles
Nutrition
Metabolism
Insulin Resistance
Adipose Tissue
Body Mass Index
Aging of materials
Exercise
Insulin
mechanistic target of rapamycin complex 1
Proteins

Keywords

  • Aging
  • Gender
  • MTOR
  • Protein metabolism
  • Sarcopenia
  • Stable isotopes

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

Cite this

Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women. / Markofski, Melissa M.; Dickinson, Jared M.; Drummond, Micah J.; Fry, Christopher; Fujita, Satoshi; Gundermann, David M.; Glynn, Erin L.; Jennings, Kristofer; Paddon-Jones, Douglas; Reidy, Paul T.; Sheffield-Moore, Melinda; Timmerman, Kyle L.; Rasmussen, Blake; Volpi, Elena.

In: Experimental Gerontology, Vol. 65, 01.05.2015, p. 1-7.

Research output: Contribution to journalArticle

Markofski, MM, Dickinson, JM, Drummond, MJ, Fry, C, Fujita, S, Gundermann, DM, Glynn, EL, Jennings, K, Paddon-Jones, D, Reidy, PT, Sheffield-Moore, M, Timmerman, KL, Rasmussen, B & Volpi, E 2015, 'Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women', Experimental Gerontology, vol. 65, pp. 1-7. https://doi.org/10.1016/j.exger.2015.02.015
Markofski, Melissa M. ; Dickinson, Jared M. ; Drummond, Micah J. ; Fry, Christopher ; Fujita, Satoshi ; Gundermann, David M. ; Glynn, Erin L. ; Jennings, Kristofer ; Paddon-Jones, Douglas ; Reidy, Paul T. ; Sheffield-Moore, Melinda ; Timmerman, Kyle L. ; Rasmussen, Blake ; Volpi, Elena. / Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women. In: Experimental Gerontology. 2015 ; Vol. 65. pp. 1-7.
@article{ce3cfd6b52164683b5083b6272d87aff,
title = "Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women",
abstract = "The rate of muscle loss with aging is higher in men than women. However, women have smaller muscles throughout the adult life. Protein content is a major determinant of skeletal muscle size. This study was designed to determine if age and sex differentially impact basal muscle protein synthesis and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling. We performed a secondary data analysis on a cohort of 215 healthy, non-obese (BMI<30kg·m-2) young (18-40y; 74 men, 52 women) and older (60-87y; 57 men, 32 women) adults. The database contained information on physical characteristics, basal muscle protein fractional synthetic rate (FSR; n=215; stable isotope methodology) and mTORC1 signaling (n=125, Western blotting). FSR and mTORC1 signaling were measured at rest and after an overnight fast. mTORC1 and S6K1 phosphorylation were higher (p<0.05) in older subjects with no sex differences. However, there were no age or sex differences or interaction for muscle FSR (p>0.05). Body mass index, fat free mass, or body fat was not a significant covariate and did not influence the results. We conclude that age and sex do not influence basal muscle protein synthesis. However, basal mTORC1 hyperphosphorylation in the elderly may contribute to insulin resistance and the age-related anabolic resistance of skeletal muscle protein metabolism to nutrition and exercise.",
keywords = "Aging, Gender, MTOR, Protein metabolism, Sarcopenia, Stable isotopes",
author = "Markofski, {Melissa M.} and Dickinson, {Jared M.} and Drummond, {Micah J.} and Christopher Fry and Satoshi Fujita and Gundermann, {David M.} and Glynn, {Erin L.} and Kristofer Jennings and Douglas Paddon-Jones and Reidy, {Paul T.} and Melinda Sheffield-Moore and Timmerman, {Kyle L.} and Blake Rasmussen and Elena Volpi",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.exger.2015.02.015",
language = "English (US)",
volume = "65",
pages = "1--7",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women

AU - Markofski, Melissa M.

AU - Dickinson, Jared M.

AU - Drummond, Micah J.

AU - Fry, Christopher

AU - Fujita, Satoshi

AU - Gundermann, David M.

AU - Glynn, Erin L.

AU - Jennings, Kristofer

AU - Paddon-Jones, Douglas

AU - Reidy, Paul T.

AU - Sheffield-Moore, Melinda

AU - Timmerman, Kyle L.

AU - Rasmussen, Blake

AU - Volpi, Elena

PY - 2015/5/1

Y1 - 2015/5/1

N2 - The rate of muscle loss with aging is higher in men than women. However, women have smaller muscles throughout the adult life. Protein content is a major determinant of skeletal muscle size. This study was designed to determine if age and sex differentially impact basal muscle protein synthesis and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling. We performed a secondary data analysis on a cohort of 215 healthy, non-obese (BMI<30kg·m-2) young (18-40y; 74 men, 52 women) and older (60-87y; 57 men, 32 women) adults. The database contained information on physical characteristics, basal muscle protein fractional synthetic rate (FSR; n=215; stable isotope methodology) and mTORC1 signaling (n=125, Western blotting). FSR and mTORC1 signaling were measured at rest and after an overnight fast. mTORC1 and S6K1 phosphorylation were higher (p<0.05) in older subjects with no sex differences. However, there were no age or sex differences or interaction for muscle FSR (p>0.05). Body mass index, fat free mass, or body fat was not a significant covariate and did not influence the results. We conclude that age and sex do not influence basal muscle protein synthesis. However, basal mTORC1 hyperphosphorylation in the elderly may contribute to insulin resistance and the age-related anabolic resistance of skeletal muscle protein metabolism to nutrition and exercise.

AB - The rate of muscle loss with aging is higher in men than women. However, women have smaller muscles throughout the adult life. Protein content is a major determinant of skeletal muscle size. This study was designed to determine if age and sex differentially impact basal muscle protein synthesis and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling. We performed a secondary data analysis on a cohort of 215 healthy, non-obese (BMI<30kg·m-2) young (18-40y; 74 men, 52 women) and older (60-87y; 57 men, 32 women) adults. The database contained information on physical characteristics, basal muscle protein fractional synthetic rate (FSR; n=215; stable isotope methodology) and mTORC1 signaling (n=125, Western blotting). FSR and mTORC1 signaling were measured at rest and after an overnight fast. mTORC1 and S6K1 phosphorylation were higher (p<0.05) in older subjects with no sex differences. However, there were no age or sex differences or interaction for muscle FSR (p>0.05). Body mass index, fat free mass, or body fat was not a significant covariate and did not influence the results. We conclude that age and sex do not influence basal muscle protein synthesis. However, basal mTORC1 hyperphosphorylation in the elderly may contribute to insulin resistance and the age-related anabolic resistance of skeletal muscle protein metabolism to nutrition and exercise.

KW - Aging

KW - Gender

KW - MTOR

KW - Protein metabolism

KW - Sarcopenia

KW - Stable isotopes

UR - http://www.scopus.com/inward/record.url?scp=84924094844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924094844&partnerID=8YFLogxK

U2 - 10.1016/j.exger.2015.02.015

DO - 10.1016/j.exger.2015.02.015

M3 - Article

VL - 65

SP - 1

EP - 7

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

ER -