Effect of aging on neurotensin-stimulated growth of rat small intestine

B. M. Evers, M. Izukura, S. Rajaraman, D. Parekh, K. Thakore, K. Yoshinaga, T. Uchida, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The proliferative activity of gut mucosa is altered with aging; the potential for the aged gut to respond to trophic stimuli is not known. The purpose of this study was to determine whether there are age-related differences in the effects of the trophic gut peptide neurotensin (NT) on the structure and function of small bowel mucosa. NT (300 μg/kg) or saline (control) was injected subcutaneously at 8-h intervals for 5 days in rats of two age groups, young (2 mo) and aged (24 mo). On day 6, rats were killed, and the gut mucosa (proximal and distal small bowel) was scraped, weighed, and analyzed for DNA, RNA, and protein content and for disaccharidase (sucrase and maltase) activity. In a second experiment, the groups of rats and the protocol for NT administration were identical; however, when the rats were killed, the distal gut was removed for histological evaluation of crypt and villus length (mm) and density (no./cm gut segment) and bromodeoxyuridine immunohistochemistry. NT produced significant increases in mucosal growth (wt, DNA, RNA, and protein) in both age groups when compared with age- matched controls; the increase of growth measurements was the greatest in the small bowel mucosa of the aged rats. In addition, NT increased crypt density in both groups; only the aged group treated with NT demonstrated increases in crypt depth and villus height. Specific activities of sucrase and maltase did not change with NT treatment in either of the age groups. We conclude that the proliferative potential of small bowel mucosa is maintained with aging in response to administration of NT. This effect of NT is predominantly on gut mucosal structure and does not appear to affect function as measured by alterations in disaccharidase activity.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume267
Issue number2 30-2
StatePublished - 1994

Fingerprint

Neurotensin
Small Intestine
Growth
Mucous Membrane
Disaccharidases
Sucrase
alpha-Glucosidases
Age Groups
RNA
DNA
Bromodeoxyuridine
Proteins
Immunohistochemistry
Peptides

Keywords

  • small bowel mucosa

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Evers, B. M., Izukura, M., Rajaraman, S., Parekh, D., Thakore, K., Yoshinaga, K., ... Thompson, J. C. (1994). Effect of aging on neurotensin-stimulated growth of rat small intestine. American Journal of Physiology - Gastrointestinal and Liver Physiology, 267(2 30-2).

Effect of aging on neurotensin-stimulated growth of rat small intestine. / Evers, B. M.; Izukura, M.; Rajaraman, S.; Parekh, D.; Thakore, K.; Yoshinaga, K.; Uchida, T.; Townsend, Courtney; Thompson, J. C.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 267, No. 2 30-2, 1994.

Research output: Contribution to journalArticle

Evers, BM, Izukura, M, Rajaraman, S, Parekh, D, Thakore, K, Yoshinaga, K, Uchida, T, Townsend, C & Thompson, JC 1994, 'Effect of aging on neurotensin-stimulated growth of rat small intestine', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 267, no. 2 30-2.
Evers BM, Izukura M, Rajaraman S, Parekh D, Thakore K, Yoshinaga K et al. Effect of aging on neurotensin-stimulated growth of rat small intestine. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1994;267(2 30-2).
Evers, B. M. ; Izukura, M. ; Rajaraman, S. ; Parekh, D. ; Thakore, K. ; Yoshinaga, K. ; Uchida, T. ; Townsend, Courtney ; Thompson, J. C. / Effect of aging on neurotensin-stimulated growth of rat small intestine. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1994 ; Vol. 267, No. 2 30-2.
@article{3bac988f69364a28a3c2a4d3f5312c71,
title = "Effect of aging on neurotensin-stimulated growth of rat small intestine",
abstract = "The proliferative activity of gut mucosa is altered with aging; the potential for the aged gut to respond to trophic stimuli is not known. The purpose of this study was to determine whether there are age-related differences in the effects of the trophic gut peptide neurotensin (NT) on the structure and function of small bowel mucosa. NT (300 μg/kg) or saline (control) was injected subcutaneously at 8-h intervals for 5 days in rats of two age groups, young (2 mo) and aged (24 mo). On day 6, rats were killed, and the gut mucosa (proximal and distal small bowel) was scraped, weighed, and analyzed for DNA, RNA, and protein content and for disaccharidase (sucrase and maltase) activity. In a second experiment, the groups of rats and the protocol for NT administration were identical; however, when the rats were killed, the distal gut was removed for histological evaluation of crypt and villus length (mm) and density (no./cm gut segment) and bromodeoxyuridine immunohistochemistry. NT produced significant increases in mucosal growth (wt, DNA, RNA, and protein) in both age groups when compared with age- matched controls; the increase of growth measurements was the greatest in the small bowel mucosa of the aged rats. In addition, NT increased crypt density in both groups; only the aged group treated with NT demonstrated increases in crypt depth and villus height. Specific activities of sucrase and maltase did not change with NT treatment in either of the age groups. We conclude that the proliferative potential of small bowel mucosa is maintained with aging in response to administration of NT. This effect of NT is predominantly on gut mucosal structure and does not appear to affect function as measured by alterations in disaccharidase activity.",
keywords = "small bowel mucosa",
author = "Evers, {B. M.} and M. Izukura and S. Rajaraman and D. Parekh and K. Thakore and K. Yoshinaga and T. Uchida and Courtney Townsend and Thompson, {J. C.}",
year = "1994",
language = "English",
volume = "267",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "2 30-2",

}

TY - JOUR

T1 - Effect of aging on neurotensin-stimulated growth of rat small intestine

AU - Evers, B. M.

AU - Izukura, M.

AU - Rajaraman, S.

AU - Parekh, D.

AU - Thakore, K.

AU - Yoshinaga, K.

AU - Uchida, T.

AU - Townsend, Courtney

AU - Thompson, J. C.

PY - 1994

Y1 - 1994

N2 - The proliferative activity of gut mucosa is altered with aging; the potential for the aged gut to respond to trophic stimuli is not known. The purpose of this study was to determine whether there are age-related differences in the effects of the trophic gut peptide neurotensin (NT) on the structure and function of small bowel mucosa. NT (300 μg/kg) or saline (control) was injected subcutaneously at 8-h intervals for 5 days in rats of two age groups, young (2 mo) and aged (24 mo). On day 6, rats were killed, and the gut mucosa (proximal and distal small bowel) was scraped, weighed, and analyzed for DNA, RNA, and protein content and for disaccharidase (sucrase and maltase) activity. In a second experiment, the groups of rats and the protocol for NT administration were identical; however, when the rats were killed, the distal gut was removed for histological evaluation of crypt and villus length (mm) and density (no./cm gut segment) and bromodeoxyuridine immunohistochemistry. NT produced significant increases in mucosal growth (wt, DNA, RNA, and protein) in both age groups when compared with age- matched controls; the increase of growth measurements was the greatest in the small bowel mucosa of the aged rats. In addition, NT increased crypt density in both groups; only the aged group treated with NT demonstrated increases in crypt depth and villus height. Specific activities of sucrase and maltase did not change with NT treatment in either of the age groups. We conclude that the proliferative potential of small bowel mucosa is maintained with aging in response to administration of NT. This effect of NT is predominantly on gut mucosal structure and does not appear to affect function as measured by alterations in disaccharidase activity.

AB - The proliferative activity of gut mucosa is altered with aging; the potential for the aged gut to respond to trophic stimuli is not known. The purpose of this study was to determine whether there are age-related differences in the effects of the trophic gut peptide neurotensin (NT) on the structure and function of small bowel mucosa. NT (300 μg/kg) or saline (control) was injected subcutaneously at 8-h intervals for 5 days in rats of two age groups, young (2 mo) and aged (24 mo). On day 6, rats were killed, and the gut mucosa (proximal and distal small bowel) was scraped, weighed, and analyzed for DNA, RNA, and protein content and for disaccharidase (sucrase and maltase) activity. In a second experiment, the groups of rats and the protocol for NT administration were identical; however, when the rats were killed, the distal gut was removed for histological evaluation of crypt and villus length (mm) and density (no./cm gut segment) and bromodeoxyuridine immunohistochemistry. NT produced significant increases in mucosal growth (wt, DNA, RNA, and protein) in both age groups when compared with age- matched controls; the increase of growth measurements was the greatest in the small bowel mucosa of the aged rats. In addition, NT increased crypt density in both groups; only the aged group treated with NT demonstrated increases in crypt depth and villus height. Specific activities of sucrase and maltase did not change with NT treatment in either of the age groups. We conclude that the proliferative potential of small bowel mucosa is maintained with aging in response to administration of NT. This effect of NT is predominantly on gut mucosal structure and does not appear to affect function as measured by alterations in disaccharidase activity.

KW - small bowel mucosa

UR - http://www.scopus.com/inward/record.url?scp=0028040876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028040876&partnerID=8YFLogxK

M3 - Article

VL - 267

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 2 30-2

ER -