Effect of antioxidant treatment on spinal GABA neurons in a neuropathic pain model in the mouse

June Guillet, Jigong Wang, Hee Young Kim, Ying Lu, Kyungsoon Chung, Jin Chung

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

One feature of neuropathic pain is a reduced spinal gamma-aminobutyric acid (GABA)-ergic inhibitory function. However, the mechanisms behind this attenuation remain to be elucidated. This study investigated the involvement of reactive oxygen species in the spinal GABA neuron loss and reduced GABA neuron excitability in spinal nerve ligation (SNL) model of neuropathic pain in mice. The importance of spinal GABAergic inhibition in neuropathic pain was tested by examining the effects of intrathecally administered GABA receptor agonists and antagonists in SNL and naïve mice, respectively. The effects of SNL and antioxidant treatment on GABA neuron loss and functional changes were examined in transgenic GAD67-enhanced green fluorescent protein positive (EGFP+) mice. GABA receptor agonists transiently reversed mechanical hypersensitivity of the hind paw in SNL mice. On the other hand, GABA receptor antagonists made naïve mice mechanically hypersensitive. Stereological analysis showed that the numbers of enhanced green fluorescent protein positive (EGFP+) GABA neurons were significantly decreased in the lateral superficial laminae (I-II) on the ipsilateral L5 spinal cord after SNL. Repeated antioxidant treatments significantly reduced the pain behaviors and prevented the reduction in EGFP+ GABA neurons. The response rate of the tonic firing GABA neurons recorded from SNL mice increased with antioxidant treatment, whereas no change was seen in those recorded from naïve mice, which suggested that oxidative stress impaired some spinal GABA neuron activity in the neuropathic pain condition. Together the data suggest that neuropathic pain, at least partially, is attributed to oxidative stress, which induces both a GABA neuron loss and dysfunction of surviving GABA neurons.

Original languageEnglish (US)
Pages (from-to)2469-2476
Number of pages8
JournalPain
Volume154
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Neuralgia
gamma-Aminobutyric Acid
Antioxidants
Spinal Nerves
Neurons
Ligation
GABA Receptors
GABA Antagonists
GABA Agonists
Therapeutics
Oxidative Stress
Reactive Oxygen Species
Spinal Cord
Hypersensitivity
Pain

Keywords

  • Free radicals
  • GABA
  • Neuropathic pain
  • Reactive oxygen species
  • Spinal cord dorsal horn neurons

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology
  • Medicine(all)

Cite this

Effect of antioxidant treatment on spinal GABA neurons in a neuropathic pain model in the mouse. / Guillet, June; Wang, Jigong; Kim, Hee Young; Lu, Ying; Chung, Kyungsoon; Chung, Jin.

In: Pain, Vol. 154, No. 11, 11.2013, p. 2469-2476.

Research output: Contribution to journalArticle

Guillet, June ; Wang, Jigong ; Kim, Hee Young ; Lu, Ying ; Chung, Kyungsoon ; Chung, Jin. / Effect of antioxidant treatment on spinal GABA neurons in a neuropathic pain model in the mouse. In: Pain. 2013 ; Vol. 154, No. 11. pp. 2469-2476.
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