Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria

V. Pérez-Vázquez, J. Ramírez, L. Aguilera-Aguirre, J. C. González-Hernández, M. Clemente-Guerrero, S. Manzo-Ávalos, S. Uribe, A. Saavedra-Molina

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

Original languageEnglish (US)
Pages (from-to)405-416
Number of pages12
JournalAmino Acids
Volume22
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Heart Mitochondria
Mitochondria
Liver Mitochondrion
Liver
Superoxide Dismutase
Rats
Oxygen
Oxides
Hydrogen Peroxide
Anions
Tissue
Oxidative Stress
Oxidative stress
Metabolism
Enzymes
Chemical activation

Keywords

  • Amino acids
  • Calcium
  • Heart mitochondria
  • Liver mitochondria
  • Magnesium
  • Mn-SOD
  • Superoxide anion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

Pérez-Vázquez, V., Ramírez, J., Aguilera-Aguirre, L., González-Hernández, J. C., Clemente-Guerrero, M., Manzo-Ávalos, S., ... Saavedra-Molina, A. (2002). Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria. Amino Acids, 22(4), 405-416. https://doi.org/10.1007/s007260200024

Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria. / Pérez-Vázquez, V.; Ramírez, J.; Aguilera-Aguirre, L.; González-Hernández, J. C.; Clemente-Guerrero, M.; Manzo-Ávalos, S.; Uribe, S.; Saavedra-Molina, A.

In: Amino Acids, Vol. 22, No. 4, 2002, p. 405-416.

Research output: Contribution to journalArticle

Pérez-Vázquez, V, Ramírez, J, Aguilera-Aguirre, L, González-Hernández, JC, Clemente-Guerrero, M, Manzo-Ávalos, S, Uribe, S & Saavedra-Molina, A 2002, 'Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria', Amino Acids, vol. 22, no. 4, pp. 405-416. https://doi.org/10.1007/s007260200024
Pérez-Vázquez V, Ramírez J, Aguilera-Aguirre L, González-Hernández JC, Clemente-Guerrero M, Manzo-Ávalos S et al. Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria. Amino Acids. 2002;22(4):405-416. https://doi.org/10.1007/s007260200024
Pérez-Vázquez, V. ; Ramírez, J. ; Aguilera-Aguirre, L. ; González-Hernández, J. C. ; Clemente-Guerrero, M. ; Manzo-Ávalos, S. ; Uribe, S. ; Saavedra-Molina, A. / Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria. In: Amino Acids. 2002 ; Vol. 22, No. 4. pp. 405-416.
@article{47f8747d13ea40d499c57ad4ce4a9f8c,
title = "Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria",
abstract = "The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.",
keywords = "Amino acids, Calcium, Heart mitochondria, Liver mitochondria, Magnesium, Mn-SOD, Superoxide anion",
author = "V. P{\'e}rez-V{\'a}zquez and J. Ram{\'i}rez and L. Aguilera-Aguirre and Gonz{\'a}lez-Hern{\'a}ndez, {J. C.} and M. Clemente-Guerrero and S. Manzo-{\'A}valos and S. Uribe and A. Saavedra-Molina",
year = "2002",
doi = "10.1007/s007260200024",
language = "English (US)",
volume = "22",
pages = "405--416",
journal = "Amino Acids",
issn = "0939-4451",
publisher = "Springer Wien",
number = "4",

}

TY - JOUR

T1 - Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria

AU - Pérez-Vázquez, V.

AU - Ramírez, J.

AU - Aguilera-Aguirre, L.

AU - González-Hernández, J. C.

AU - Clemente-Guerrero, M.

AU - Manzo-Ávalos, S.

AU - Uribe, S.

AU - Saavedra-Molina, A.

PY - 2002

Y1 - 2002

N2 - The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

AB - The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

KW - Amino acids

KW - Calcium

KW - Heart mitochondria

KW - Liver mitochondria

KW - Magnesium

KW - Mn-SOD

KW - Superoxide anion

UR - http://www.scopus.com/inward/record.url?scp=0036278233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036278233&partnerID=8YFLogxK

U2 - 10.1007/s007260200024

DO - 10.1007/s007260200024

M3 - Article

C2 - 12107766

AN - SCOPUS:0036278233

VL - 22

SP - 405

EP - 416

JO - Amino Acids

JF - Amino Acids

SN - 0939-4451

IS - 4

ER -