Effect of calcitonin gene-related peptide on glucose and gastric inhibitory polypeptide-stimulated insulin release from cultured newborn and adult rat islet cells

Jin Ishizuka, George H. Greeley, Cary W. Cooper, James C. Thompson

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Calcitonin gene-related peptide (CGRP) is a 37-amino acid peptide that is present in peripheral cells of islets and in nerves around and within islets. CGRP can inhibit insulin secretion in vitro and in vivo. Whether the inhibitory action of CGRP is mediated by somatostatin or by nerve terminals is, however, not known. The objective of this study was to examine the effect of CGRP on insulin secretion, using cultured newborn and adult rat islet cells which did not contain nerve terminals. In adult rat islet cells, CGRP (10-10 to 10-8 M) significantly inhibited glucose-stimulated and gastric inhibitory polypeptide (GIP)-potentiated insulin secretion, but in newborn rat islet cells, CGRP did not inhibit glucose-stimulated insulin secretion. Inhibition of glucose-stimulated and GIP-potentiated insulin release was dependent on the glucose concentration during the prestimulation period. CGRP did not stimulate release of somatostatin. These findings suggest that rat CGRP can act directly on β-cells through a specific receptor that is absent in newborn rat β-cells.

Original languageEnglish (US)
Pages (from-to)73-82
Number of pages10
JournalRegulatory Peptides
Volume20
Issue number1
DOIs
StatePublished - Jan 1988
Externally publishedYes

Keywords

  • Somatostatin
  • β-Cell

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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