Effect of calcitonin gene-related peptide on glucose and gastric inhibitory polypeptide-stimulated insulin release from cultured newborn and adult rat islet cells

Jin Ishizuka, George H. Greeley, Cary W. Cooper, James C. Thompson

    Research output: Contribution to journalArticle

    28 Scopus citations

    Abstract

    Calcitonin gene-related peptide (CGRP) is a 37-amino acid peptide that is present in peripheral cells of islets and in nerves around and within islets. CGRP can inhibit insulin secretion in vitro and in vivo. Whether the inhibitory action of CGRP is mediated by somatostatin or by nerve terminals is, however, not known. The objective of this study was to examine the effect of CGRP on insulin secretion, using cultured newborn and adult rat islet cells which did not contain nerve terminals. In adult rat islet cells, CGRP (10-10 to 10-8 M) significantly inhibited glucose-stimulated and gastric inhibitory polypeptide (GIP)-potentiated insulin secretion, but in newborn rat islet cells, CGRP did not inhibit glucose-stimulated insulin secretion. Inhibition of glucose-stimulated and GIP-potentiated insulin release was dependent on the glucose concentration during the prestimulation period. CGRP did not stimulate release of somatostatin. These findings suggest that rat CGRP can act directly on β-cells through a specific receptor that is absent in newborn rat β-cells.

    Original languageEnglish (US)
    Pages (from-to)73-82
    Number of pages10
    JournalRegulatory Peptides
    Volume20
    Issue number1
    DOIs
    StatePublished - Jan 1988

    Keywords

    • Somatostatin
    • β-Cell

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Clinical Biochemistry
    • Cellular and Molecular Neuroscience

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