Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria

V. Pérez-Vázquez; J. Ramírez; L. Aguilera-Aguirre; J. C. González-Hernández; M. Clemente-Guerrero; S. Manzo-Ávalos; S. Uribe; A. Saavedra-Molina

doi:10.1007/s007260200024

Effect of Ca²⁺ and Mg²⁺ on the Mn-superoxide dismutase from rat liver and heart mitochondria

V. Pérez-Vázquez, J. Ramírez, L. Aguilera-Aguirre, J. C. González-Hernández, M. Clemente-Guerrero, S. Manzo-Ávalos, S. Uribe, A. Saavedra-Molina

Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca²⁺ ([Ca²⁺]_f) and free Mg²⁺ ([Mg²⁺]_f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca²⁺]_f and [Mg²⁺]_f activated the enzyme, although [Mg²⁺]_f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca²⁺]_f and [Mg²⁺]_f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca²⁺]_f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

Original language	English (US)
Pages (from-to)	405-416
Number of pages	12
Journal	Amino Acids
Volume	22
Issue number	4
DOIs	https://doi.org/10.1007/s007260200024
State	Published - 2002

Keywords

Amino acids
Calcium
Heart mitochondria
Liver mitochondria
Magnesium
Mn-SOD
Superoxide anion

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1007/s007260200024

Cite this

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title = "Effect of Ca2+ and Mg2+ on the Mn-superoxide dismutase from rat liver and heart mitochondria",

abstract = "The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.",

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AU - Pérez-Vázquez, V.

AU - Ramírez, J.

AU - Aguilera-Aguirre, L.

AU - González-Hernández, J. C.

AU - Clemente-Guerrero, M.

AU - Manzo-Ávalos, S.

AU - Uribe, S.

AU - Saavedra-Molina, A.

PY - 2002

Y1 - 2002

N2 - The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

AB - The manganese superoxide dismutase (Mn-SOD) converts super-oxide anions to hydrogen peroxide plus oxygen, providing the first line of defense against oxidative stress in mitochondria. Heart mitochondria exhibited higher Mn-SOD activity than liver mitochondria. In mitochondria from both tissues Mn-SOD activity decreased after incubation at low oxygen concentration (hypoxic mitochondria). The effects of free Ca2+ ([Ca2+]f) and free Mg2+ ([Mg2+]f) on normoxic and hypoxic mitochondria from either organ were tested. In normoxic mitochondria from either tissue, both [Ca2+]f and [Mg2+]f activated the enzyme, although [Mg2+]f was less efficient as an activator and the effect was lower in heart than in liver mitochondria. When added simultaneously, high [Ca2+]f and [Mg2+]f exhibited additive effects which were more pronounced in heart mitochondria and were observed regardless of whether mitochondria had been incubated under normal or low oxygen. The data suggest that [Ca2+]f plays a role in regulating Mn-SOD in concert with the activation of aerobic metabolism.

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