Effect of CCL2 antisense oligodeoxynucleotides on bacterial translocation and subsequent sepsis in severely burned mice orally infected with Enterococcus faecalis

Kenji Shigematsu, Mari Kogiso, Makiko Kobayashi, David Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Severely burned mice are susceptible to sepsis stemming from Enterococcus faecalis translocation due to the impaired generation of M1 macrophages (M1Mφ{symbol}s) in local translocation sites. In our previous studies, CCL2 has been characterized as a major effector molecule on the burn-associated generation of M2Mφ{symbol}s, an inhibitor cell type for resident Mφ{symbol} conversion into M1Mφ{symbol}s. In this study, we tried to protect burned mice orally infected with E. faecalis utilizing CCL2 antisense oligodeoxynucleotides (ODNs). We show that M2Mφ{symbol}s in mesenteric lymph nodes (MLNs) were not demonstrated in burned mice treated with CCL2 antisense ODNs. M1Mφ{symbol}s were not induced by heat-killed E. faecalis from resident Mφ{symbol}s transwell-cultured with mesenteric lymph node macrophages (MLN-Mφ{symbol}s) from burned mice, while M1Mφ{symbol}s were induced by the same antigen from resident Mφ{symbol}s transwell-cultured with Mφ{symbol}s which were isolated from burned mice treated with CCL2 antisense ODNs. Bacterial growth in MLNs was shown in burned mice orally infected with a lethal dose of E. faecalis. However, after the same infection, sepsis did not develop in burned mice treated with CCL2 antisense ODNs. These results indicate that bacterial translocation and subsequent sepsis are controlled in burned mice orally infected with a lethal dose of E. faecalis by gene therapy utilizing CCL2 antisense ODNs.

Original languageEnglish (US)
Pages (from-to)158-164
Number of pages7
JournalEuropean Journal of Immunology
Volume42
Issue number1
DOIs
StatePublished - Jan 2012

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Bacterial Translocation
Enterococcus faecalis
Oligodeoxyribonucleotides
Sepsis
Lymph Nodes
Macrophages
Burns
Genetic Therapy
Hot Temperature
Antigens

Keywords

  • Bacterial translocation
  • Burn
  • CCL2
  • Immunomodulation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Effect of CCL2 antisense oligodeoxynucleotides on bacterial translocation and subsequent sepsis in severely burned mice orally infected with Enterococcus faecalis. / Shigematsu, Kenji; Kogiso, Mari; Kobayashi, Makiko; Herndon, David; Suzuki, Fujio.

In: European Journal of Immunology, Vol. 42, No. 1, 01.2012, p. 158-164.

Research output: Contribution to journalArticle

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