Effect of cefazolin and vancomycin on osteoblasts in vitro

Matthew L. Edin, Theodore Miclau, Gayle E. Lester, Ronald W. Lindsey, Laurence E. Dahners

Research output: Contribution to journalArticle

166 Scopus citations

Abstract

The effect of cefazolin and vancomycin on osteoblast-like cells was studied. Cells from the MG-63 human osteosarcoma cell line were grown in antibiotic free media and exposed to concentrations of cefazolin and vancomycin at order of magnitude intervals between 0 and 10,000 μg/ml. For cefazolin, a second interval was performed between 100 and 1000 μg/ml to define toxic levels more accurately. Cell number and 3H-thymidine incorporation at 0, 24, and 72 hours were determined. The results of this study show that local levels of vancomycin of 1000 μg/ml and less have little or no effect on osteoblast replication, and concentrations of 10,000 μg/ml cause cell death. Concentrations of cefazolin of 100 μg/ml and less have little or no effect on osteoblast replication, 200 μg/ml significantly decrease cell replication, and 10,000 μg/ml cause cell death. The authors conclude that vancomycin is less toxic than is cefazolin to osteoblasts at higher concentrations and may be a better antibiotic for local administration in the treatment of similarly sensitive bacterial infections.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalClinical orthopaedics and related research
Issue number333
DOIs
StatePublished - Dec 1996
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

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