Effect of cefazolin and vancomycin on osteoblasts in vitro

Matthew L. Edin, Theodore Miclau, Gayle E. Lester, Ronald Lindsey, Laurence E. Dahners

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

The effect of cefazolin and vancomycin on osteoblast-like cells was studied. Cells from the MG-63 human osteosarcoma cell line were grown in antibiotic free media and exposed to concentrations of cefazolin and vancomycin at order of magnitude intervals between 0 and 10,000 μg/ml. For cefazolin, a second interval was performed between 100 and 1000 μg/ml to define toxic levels more accurately. Cell number and 3H-thymidine incorporation at 0, 24, and 72 hours were determined. The results of this study show that local levels of vancomycin of 1000 μg/ml and less have little or no effect on osteoblast replication, and concentrations of 10,000 μg/ml cause cell death. Concentrations of cefazolin of 100 μg/ml and less have little or no effect on osteoblast replication, 200 μg/ml significantly decrease cell replication, and 10,000 μg/ml cause cell death. The authors conclude that vancomycin is less toxic than is cefazolin to osteoblasts at higher concentrations and may be a better antibiotic for local administration in the treatment of similarly sensitive bacterial infections.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalClinical Orthopaedics and Related Research
Issue number333
StatePublished - Dec 1996
Externally publishedYes

Fingerprint

Cefazolin
Vancomycin
Osteoblasts
Poisons
Cause of Death
Cell Death
Anti-Bacterial Agents
Osteosarcoma
Bacterial Infections
Thymidine
Cell Count
In Vitro Techniques
Cell Line

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Edin, M. L., Miclau, T., Lester, G. E., Lindsey, R., & Dahners, L. E. (1996). Effect of cefazolin and vancomycin on osteoblasts in vitro. Clinical Orthopaedics and Related Research, (333), 245-251.

Effect of cefazolin and vancomycin on osteoblasts in vitro. / Edin, Matthew L.; Miclau, Theodore; Lester, Gayle E.; Lindsey, Ronald; Dahners, Laurence E.

In: Clinical Orthopaedics and Related Research, No. 333, 12.1996, p. 245-251.

Research output: Contribution to journalArticle

Edin, ML, Miclau, T, Lester, GE, Lindsey, R & Dahners, LE 1996, 'Effect of cefazolin and vancomycin on osteoblasts in vitro', Clinical Orthopaedics and Related Research, no. 333, pp. 245-251.
Edin, Matthew L. ; Miclau, Theodore ; Lester, Gayle E. ; Lindsey, Ronald ; Dahners, Laurence E. / Effect of cefazolin and vancomycin on osteoblasts in vitro. In: Clinical Orthopaedics and Related Research. 1996 ; No. 333. pp. 245-251.
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