Effect of ciprofloxacin on the proliferation of osteoblast-like MG-63 human osteosarcoma cells in vitro

T. Miclau, M. L. Edin, G. E. Lester, Ronald Lindsey, L. E. Dahners

Research output: Contribution to journalArticle

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Abstract

Locally applied antibiotic therapy is gaining popularity for the treatment of infections associated with open fractures and posttraumatic osteomyelitis. With use of local techniques, ciprofloxacin levels as high as 1,300 μg/ml, or over 200 times the bone levels achieved with intravenous administration, have been re ported. To study the possible effects of ciprofloxacin on bone, osteoblast-like cells from the MG-63 human osteosarcoma cell line were studied. The cells were grown in antibiotic-free media and exposed to concentrations of ciprofloxacin at 0, 10, 100, 200, and 1,000 μg/ml to establish an initial dose-response curve. Media containing the appropriate dose of ciprofloxacin were changed every 24 hours. Cell number and [3H]thymidine incorporation per cell were determined at 0, 24, and 72 hours. A second dose-response curve was per formed at concentrations of 0, 10, 20, 40, and 80 μg/ml. Three experiments, each with four observations, were performed. The results of this study demonstrated that ciprofloxacin caused significant decreases (p < 0.05) in cell number at 40 μg/ml at 24 hours and 20 μg/ml at 72 hours. [3H]thymidine incorporation per cell decreased significantly at levels of 80 μg/ml at 24 hours and 20 μg/ml at 72 hours. The authors conclude that reported local levels of ciprofloxacin seen in vivo inhibit the proliferation of human osteoblast- like cells in vitro.

Original languageEnglish (US)
Pages (from-to)509-512
Number of pages4
JournalJournal of Orthopaedic Research
Volume16
Issue number4
DOIs
StatePublished - Jul 1998
Externally publishedYes

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Osteosarcoma
Ciprofloxacin
Osteoblasts
Thymidine
Cell Count
Anti-Bacterial Agents
Bone and Bones
Open Fractures
Osteomyelitis
Intravenous Administration
In Vitro Techniques
Cell Line
Therapeutics
Infection

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Effect of ciprofloxacin on the proliferation of osteoblast-like MG-63 human osteosarcoma cells in vitro. / Miclau, T.; Edin, M. L.; Lester, G. E.; Lindsey, Ronald; Dahners, L. E.

In: Journal of Orthopaedic Research, Vol. 16, No. 4, 07.1998, p. 509-512.

Research output: Contribution to journalArticle

Miclau, T. ; Edin, M. L. ; Lester, G. E. ; Lindsey, Ronald ; Dahners, L. E. / Effect of ciprofloxacin on the proliferation of osteoblast-like MG-63 human osteosarcoma cells in vitro. In: Journal of Orthopaedic Research. 1998 ; Vol. 16, No. 4. pp. 509-512.
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