Effect of cyclic nucleotides on bombesin-evoked gastrin release from isolated perfused rat stomach

Yan Shi Guo, Vinod K. Bhalla, James C. Thompson, Pomila Singh

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We and others have recently reported an involvement of calcium (Ca2+)-mediated intracellular pathways in the release of antral gastrin in response to bombesin (BBS), while cyclic adenosine 3′5′-monophosphate (cAMP) potentiated the gastrin response to BBS. In this study we examined the effect of cyclic nucleotides on BBS-induced gastrin release from isolated perfused rat stomachs. Dibutyryl cyclic AMP (dbcAMP, 1 mM), and Rolipram (a phosphodiesterase inhibitor, 0.5 μM), stimulated basal gastrin secretion and potentiated BBS-induced gastrin release. The stimulation of gastrin release by BBS was not altered by Wiptide (a cAMP dependent protein kinase inhibitor, 1.0 μM), but was surprisingly inhibited by dbcGMP (1 mM). The cAMP content in antral mucosa or in the perfusates was not changed after infusion of BBS. These findings coupled with previous results suggest that BBS-provoked gastrin release is principally coupled to a Ca2+-mediated intracellular pathway, and that an activation of the adenylate cyclase mediated pathway is not involved. Intracellular cGMP, however, may participate in the negative regulation of gastrin release induced by BBS.

Original languageEnglish (US)
Pages (from-to)361-369
Number of pages9
JournalRegulatory Peptides
Volume22
Issue number4
DOIs
StatePublished - Sep 1988

Keywords

  • Bombesin
  • Gastrin
  • Isolated rat stomach
  • cAMP
  • cGMP

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Effect of cyclic nucleotides on bombesin-evoked gastrin release from isolated perfused rat stomach'. Together they form a unique fingerprint.

Cite this