Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apo VLDL) II in the Liver of Quail Embryos

Kouhei Ichikawa, Yonju Ha, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II. The fertile eggs were treated with a single injection of either NP, OP or ethynyl estradiol (EE) at doses of 10 and 100 nmole/egg in 20μl on day 13 of incubation. In the control group the eggs were treated with the vehicle (corn oil, 20μl/egg). On day 15 of incubation the liver was collected and total RNA was extracted. Both mRNA levels were determined by RT-PCR assay and were expressed in relation to ø actin mRNA levels. No expression of VTG II mRNA was detected in the control group, whereas a marked induction of VTG II mRNA was revealed in the EE treatment. A weak but distinct expression of VTG II mRNA was evident in the NP and OP treatment groups. ApoVLDLII transcripts were detected in the control group and induced markedly by the injection of EE with higher expression in females. NP also induced considerable expression in females, whereas no transcripts were detected in males. OP also induced the transcript in females but in males OP at 10 nmole was effective. This study indicates that NP and OP possess estrogenic activity in terms of liver VTG II and apoVLDLII mRNA expression in the quail embryo, and that apoVLDLII expression in female embryo is more sensitive to estrogenic substances.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalJournal of Poultry Science
Volume40
Issue number1
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

endocrine-disrupting chemicals
very low density lipoprotein
vitellogenin
quails
embryo (animal)
liver
estradiol
estrogenic properties
injection
corn oil
actin
reverse transcriptase polymerase chain reaction
RNA
assays
dosage

Keywords

  • apoVLDLII
  • endocrine disrupters
  • quail
  • RT-PCR
  • VTG II

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apo VLDL) II in the Liver of Quail Embryos. / Ichikawa, Kouhei; Ha, Yonju; Tsukada, Akira; Saito, Noboru; Shimada, Kiyoshi.

In: Journal of Poultry Science, Vol. 40, No. 1, 2003, p. 45-52.

Research output: Contribution to journalArticle

@article{b3113d4d4e7f45dd83770282da2bc9bf,
title = "Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apo VLDL) II in the Liver of Quail Embryos",
abstract = "The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II. The fertile eggs were treated with a single injection of either NP, OP or ethynyl estradiol (EE) at doses of 10 and 100 nmole/egg in 20μl on day 13 of incubation. In the control group the eggs were treated with the vehicle (corn oil, 20μl/egg). On day 15 of incubation the liver was collected and total RNA was extracted. Both mRNA levels were determined by RT-PCR assay and were expressed in relation to {\o} actin mRNA levels. No expression of VTG II mRNA was detected in the control group, whereas a marked induction of VTG II mRNA was revealed in the EE treatment. A weak but distinct expression of VTG II mRNA was evident in the NP and OP treatment groups. ApoVLDLII transcripts were detected in the control group and induced markedly by the injection of EE with higher expression in females. NP also induced considerable expression in females, whereas no transcripts were detected in males. OP also induced the transcript in females but in males OP at 10 nmole was effective. This study indicates that NP and OP possess estrogenic activity in terms of liver VTG II and apoVLDLII mRNA expression in the quail embryo, and that apoVLDLII expression in female embryo is more sensitive to estrogenic substances.",
keywords = "apoVLDLII, endocrine disrupters, quail, RT-PCR, VTG II",
author = "Kouhei Ichikawa and Yonju Ha and Akira Tsukada and Noboru Saito and Kiyoshi Shimada",
year = "2003",
doi = "10.2141/jpsa.40.45",
language = "English (US)",
volume = "40",
pages = "45--52",
journal = "Journal of Poultry Science",
issn = "1346-7395",
publisher = "Japan Poultry Science Association",
number = "1",

}

TY - JOUR

T1 - Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apo VLDL) II in the Liver of Quail Embryos

AU - Ichikawa, Kouhei

AU - Ha, Yonju

AU - Tsukada, Akira

AU - Saito, Noboru

AU - Shimada, Kiyoshi

PY - 2003

Y1 - 2003

N2 - The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II. The fertile eggs were treated with a single injection of either NP, OP or ethynyl estradiol (EE) at doses of 10 and 100 nmole/egg in 20μl on day 13 of incubation. In the control group the eggs were treated with the vehicle (corn oil, 20μl/egg). On day 15 of incubation the liver was collected and total RNA was extracted. Both mRNA levels were determined by RT-PCR assay and were expressed in relation to ø actin mRNA levels. No expression of VTG II mRNA was detected in the control group, whereas a marked induction of VTG II mRNA was revealed in the EE treatment. A weak but distinct expression of VTG II mRNA was evident in the NP and OP treatment groups. ApoVLDLII transcripts were detected in the control group and induced markedly by the injection of EE with higher expression in females. NP also induced considerable expression in females, whereas no transcripts were detected in males. OP also induced the transcript in females but in males OP at 10 nmole was effective. This study indicates that NP and OP possess estrogenic activity in terms of liver VTG II and apoVLDLII mRNA expression in the quail embryo, and that apoVLDLII expression in female embryo is more sensitive to estrogenic substances.

AB - The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density lipoprotein (apoVLDL) II. The fertile eggs were treated with a single injection of either NP, OP or ethynyl estradiol (EE) at doses of 10 and 100 nmole/egg in 20μl on day 13 of incubation. In the control group the eggs were treated with the vehicle (corn oil, 20μl/egg). On day 15 of incubation the liver was collected and total RNA was extracted. Both mRNA levels were determined by RT-PCR assay and were expressed in relation to ø actin mRNA levels. No expression of VTG II mRNA was detected in the control group, whereas a marked induction of VTG II mRNA was revealed in the EE treatment. A weak but distinct expression of VTG II mRNA was evident in the NP and OP treatment groups. ApoVLDLII transcripts were detected in the control group and induced markedly by the injection of EE with higher expression in females. NP also induced considerable expression in females, whereas no transcripts were detected in males. OP also induced the transcript in females but in males OP at 10 nmole was effective. This study indicates that NP and OP possess estrogenic activity in terms of liver VTG II and apoVLDLII mRNA expression in the quail embryo, and that apoVLDLII expression in female embryo is more sensitive to estrogenic substances.

KW - apoVLDLII

KW - endocrine disrupters

KW - quail

KW - RT-PCR

KW - VTG II

UR - http://www.scopus.com/inward/record.url?scp=11244285022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11244285022&partnerID=8YFLogxK

U2 - 10.2141/jpsa.40.45

DO - 10.2141/jpsa.40.45

M3 - Article

AN - SCOPUS:11244285022

VL - 40

SP - 45

EP - 52

JO - Journal of Poultry Science

JF - Journal of Poultry Science

SN - 1346-7395

IS - 1

ER -