Effect of gestational age on in-vitro responses of pregnant rat aorta

V. Jain, Y. P. Vedernikov, George Saade, K. Chwalisz, R. E. Garfield

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The hypothesis that the changes in vascular reactivity seen during pregnancy are determined by the gestational age was examined. Experiments were designed to investigate changes in vascular responses with progression of pregnancy. The contractile responses to potassium and phenylephrine (in the presence and absence of Nω-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor) and the relaxant responses to acetylcholine and sodium nitroprusside were measured in isolated aortic rings from pregnant rats at various stages of gestation and from non-pregnant female rats. Potassium-evoked contractile response was higher early in pregnancy and was decreased at term (P < 0.05). The contractile response to phenyllephrine was decreased and the relaxant response to acetylcholine was increased in early pregnancy (P < 0.05). Inhibition of nitric oxide synthase caused an increase in the contractile response to phenylephrine in all the groups, but the attenuation of the response in early pregnancy was maintained (P < 0.05). There was a small decrease in the maximal relaxant response to sodium nitroprusside at term (P < 0.05). It was concluded that the effects of pregnancy on the responses of rat aorta in vitro vary at different stages of gestation. Vascular resistance may be lowered by changes in vascular reactivity in early gestation and by a decrease in the contractile potential of the vasculature during the later stages.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalHuman Reproduction
Volume13
Issue number1
DOIs
StatePublished - 1998

Fingerprint

Gestational Age
Aorta
Pregnancy
Blood Vessels
Nitroprusside
Phenylephrine
Nitric Oxide Synthase
Acetylcholine
Potassium
In Vitro Techniques
Vascular Resistance

Keywords

  • Endothelium
  • Nitric oxide
  • Pregnancy
  • Rat aorta
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Effect of gestational age on in-vitro responses of pregnant rat aorta. / Jain, V.; Vedernikov, Y. P.; Saade, George; Chwalisz, K.; Garfield, R. E.

In: Human Reproduction, Vol. 13, No. 1, 1998, p. 214-219.

Research output: Contribution to journalArticle

Jain, V, Vedernikov, YP, Saade, G, Chwalisz, K & Garfield, RE 1998, 'Effect of gestational age on in-vitro responses of pregnant rat aorta', Human Reproduction, vol. 13, no. 1, pp. 214-219. https://doi.org/10.1093/humrep/13.1.214
Jain, V. ; Vedernikov, Y. P. ; Saade, George ; Chwalisz, K. ; Garfield, R. E. / Effect of gestational age on in-vitro responses of pregnant rat aorta. In: Human Reproduction. 1998 ; Vol. 13, No. 1. pp. 214-219.
@article{4e38e05758fa4e50af1a33ff152b8957,
title = "Effect of gestational age on in-vitro responses of pregnant rat aorta",
abstract = "The hypothesis that the changes in vascular reactivity seen during pregnancy are determined by the gestational age was examined. Experiments were designed to investigate changes in vascular responses with progression of pregnancy. The contractile responses to potassium and phenylephrine (in the presence and absence of Nω-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor) and the relaxant responses to acetylcholine and sodium nitroprusside were measured in isolated aortic rings from pregnant rats at various stages of gestation and from non-pregnant female rats. Potassium-evoked contractile response was higher early in pregnancy and was decreased at term (P < 0.05). The contractile response to phenyllephrine was decreased and the relaxant response to acetylcholine was increased in early pregnancy (P < 0.05). Inhibition of nitric oxide synthase caused an increase in the contractile response to phenylephrine in all the groups, but the attenuation of the response in early pregnancy was maintained (P < 0.05). There was a small decrease in the maximal relaxant response to sodium nitroprusside at term (P < 0.05). It was concluded that the effects of pregnancy on the responses of rat aorta in vitro vary at different stages of gestation. Vascular resistance may be lowered by changes in vascular reactivity in early gestation and by a decrease in the contractile potential of the vasculature during the later stages.",
keywords = "Endothelium, Nitric oxide, Pregnancy, Rat aorta, Vascular smooth muscle",
author = "V. Jain and Vedernikov, {Y. P.} and George Saade and K. Chwalisz and Garfield, {R. E.}",
year = "1998",
doi = "10.1093/humrep/13.1.214",
language = "English (US)",
volume = "13",
pages = "214--219",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Effect of gestational age on in-vitro responses of pregnant rat aorta

AU - Jain, V.

AU - Vedernikov, Y. P.

AU - Saade, George

AU - Chwalisz, K.

AU - Garfield, R. E.

PY - 1998

Y1 - 1998

N2 - The hypothesis that the changes in vascular reactivity seen during pregnancy are determined by the gestational age was examined. Experiments were designed to investigate changes in vascular responses with progression of pregnancy. The contractile responses to potassium and phenylephrine (in the presence and absence of Nω-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor) and the relaxant responses to acetylcholine and sodium nitroprusside were measured in isolated aortic rings from pregnant rats at various stages of gestation and from non-pregnant female rats. Potassium-evoked contractile response was higher early in pregnancy and was decreased at term (P < 0.05). The contractile response to phenyllephrine was decreased and the relaxant response to acetylcholine was increased in early pregnancy (P < 0.05). Inhibition of nitric oxide synthase caused an increase in the contractile response to phenylephrine in all the groups, but the attenuation of the response in early pregnancy was maintained (P < 0.05). There was a small decrease in the maximal relaxant response to sodium nitroprusside at term (P < 0.05). It was concluded that the effects of pregnancy on the responses of rat aorta in vitro vary at different stages of gestation. Vascular resistance may be lowered by changes in vascular reactivity in early gestation and by a decrease in the contractile potential of the vasculature during the later stages.

AB - The hypothesis that the changes in vascular reactivity seen during pregnancy are determined by the gestational age was examined. Experiments were designed to investigate changes in vascular responses with progression of pregnancy. The contractile responses to potassium and phenylephrine (in the presence and absence of Nω-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor) and the relaxant responses to acetylcholine and sodium nitroprusside were measured in isolated aortic rings from pregnant rats at various stages of gestation and from non-pregnant female rats. Potassium-evoked contractile response was higher early in pregnancy and was decreased at term (P < 0.05). The contractile response to phenyllephrine was decreased and the relaxant response to acetylcholine was increased in early pregnancy (P < 0.05). Inhibition of nitric oxide synthase caused an increase in the contractile response to phenylephrine in all the groups, but the attenuation of the response in early pregnancy was maintained (P < 0.05). There was a small decrease in the maximal relaxant response to sodium nitroprusside at term (P < 0.05). It was concluded that the effects of pregnancy on the responses of rat aorta in vitro vary at different stages of gestation. Vascular resistance may be lowered by changes in vascular reactivity in early gestation and by a decrease in the contractile potential of the vasculature during the later stages.

KW - Endothelium

KW - Nitric oxide

KW - Pregnancy

KW - Rat aorta

KW - Vascular smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=0031932369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031932369&partnerID=8YFLogxK

U2 - 10.1093/humrep/13.1.214

DO - 10.1093/humrep/13.1.214

M3 - Article

VL - 13

SP - 214

EP - 219

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 1

ER -