Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine

Anthony Ramsanahie, Mark S. Duxbury, Tracy C. Grikscheit, Alexander Perez, David B. Rhoads, James Gardner-Thorpe, Jennifer Ogilvie, Stanley W. Ashley, Joseph P. Vacanti, Edward E. Whang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Using tissue-engineering techniques, we have developed a neointestine that regenerates the structural and dynamic features of native small intestine. In this study, we tested neointestinal responsiveness to glucagon-like peptide 2 (GLP-2). Neointestinal cysts were engineered by seeding biodegradable polymers with neonatal rat intestinal organoid units. The cysts were matured and anastomosed to the native jejunum of syngeneic adult recipients. Animals were treated with GLP-2 [Gly2] (twice daily, 1 μg/g body wt) or vehicle alone (control) for 10 days. Rats were then killed, and tissues were harvested for analysis. Na+-glucose cotransporter (SGLT1) mRNA expression was assessed with Northern blotting and in situ hybridization. SGLT1 protein was localized by using immunofluorescence. GLP-2 administration resulted in 1.8- and 1.7-fold increases (P < 0.05) in neointestinal villus height and crypt depth, respectively. GLP-2 administration also resulted in a 2.4-fold increase (P < 0.01) in neomucosal SGLT1 mRNA expression. SGLT1 mRNA expression was localized to enterocytes throughout the villi, and SGLT1 protein was localized to the brush border of enterocytes along the entire length of villi from the neointestine of GLP-2-treated animals. The response of tissue-engineered neointestine to exogenous GLP-2 includes mucosal growth and enhanced SGLT1 expression. Therefore, tissue-engineering principles may help in dissecting the regulatory mechanisms mediating complex processes in the intestinal epithelium.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume285
Issue number6 48-6
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Glucagon-Like Peptide 2
Sodium-Glucose Transporter 1
Enterocytes
Tissue Engineering
Messenger RNA
Cysts
Organoids
Jejunum
Intestinal Mucosa
Microvilli
Northern Blotting
Small Intestine
In Situ Hybridization
Fluorescent Antibody Technique
Polymers
Glucose
Growth

Keywords

  • Glucagon-like peptide-2
  • Neomucosa
  • Sodium-glucose cotransporter 1
  • Tissue engineering

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Ramsanahie, A., Duxbury, M. S., Grikscheit, T. C., Perez, A., Rhoads, D. B., Gardner-Thorpe, J., ... Whang, E. E. (2003). Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine. American Journal of Physiology - Gastrointestinal and Liver Physiology, 285(6 48-6).

Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine. / Ramsanahie, Anthony; Duxbury, Mark S.; Grikscheit, Tracy C.; Perez, Alexander; Rhoads, David B.; Gardner-Thorpe, James; Ogilvie, Jennifer; Ashley, Stanley W.; Vacanti, Joseph P.; Whang, Edward E.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 285, No. 6 48-6, 12.2003.

Research output: Contribution to journalArticle

Ramsanahie, A, Duxbury, MS, Grikscheit, TC, Perez, A, Rhoads, DB, Gardner-Thorpe, J, Ogilvie, J, Ashley, SW, Vacanti, JP & Whang, EE 2003, 'Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 285, no. 6 48-6.
Ramsanahie, Anthony ; Duxbury, Mark S. ; Grikscheit, Tracy C. ; Perez, Alexander ; Rhoads, David B. ; Gardner-Thorpe, James ; Ogilvie, Jennifer ; Ashley, Stanley W. ; Vacanti, Joseph P. ; Whang, Edward E. / Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2003 ; Vol. 285, No. 6 48-6.
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