Effect of glycosylated hemoglobin on endothelium-dependent relaxation of omental arteries from pregnant women

Y. Vedernikov, George Saade, M. Belfort, R. E. Garfield

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: This study was intended to test the hypothesis that glycosylated hemoglobin adversely affects endothelium-dependent vascular relaxation during pregnancy. STUDY DESIGN: Omental artery rings (3 mm) obtained from patients undergoing cesarean delivery at term were suspended in organ chambers for isometric tension recording in Krebs bicarbonate solution. They were bubbled with 5% carbon dioxide in air (37°C, pH 7.4). After equilibration at 1-g passive tension and contraction with endothelin 1, concentration-relaxation curves in response to bradykinin were determined in the presence or absence of oxyhemoglobin, glycosylated hemoglobin, or nitric oxide synthase inhibitors (Nω-nitro-L-arginine methyl ester or Nω-nitro-L- arginine). RESULTS: Oxyhemoglobin and glycosylated hemoglobin attenuated the endothelium-dependent relaxation induced by bradykinin. The inhibition did not differ significantly between the 2 nitric oxide scavengers and was of the same magnitude as that noted with the nitric oxide synthase inhibitors. CONCLUSION: Glycosylation of hemoglobin does not change its effect on endothelium-dependent relaxation in human omental vessels during pregnancy. These data do not support the hypothesis that glycosylated hemoglobin may be the reason for abnormal endothelial function in diabetes.

Original languageEnglish (US)
Pages (from-to)1047-1050
Number of pages4
JournalAmerican Journal of Obstetrics and Gynecology
Volume179
Issue number4
DOIs
StatePublished - 1998

Fingerprint

Glycosylated Hemoglobin A
Endothelium
Pregnant Women
Arteries
Oxyhemoglobins
Bradykinin
Nitric Oxide Synthase
Pregnancy
Endothelin-1
Bicarbonates
Glycosylation
Vasodilation
Carbon Dioxide
Arginine
Nitric Oxide
Hemoglobins
Air

Keywords

  • Endothelium-dependent relaxation
  • Glycosylated hemoglobin
  • Human omental artery
  • Oxyhemoglobin
  • Smooth muscle

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effect of glycosylated hemoglobin on endothelium-dependent relaxation of omental arteries from pregnant women. / Vedernikov, Y.; Saade, George; Belfort, M.; Garfield, R. E.

In: American Journal of Obstetrics and Gynecology, Vol. 179, No. 4, 1998, p. 1047-1050.

Research output: Contribution to journalArticle

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AU - Garfield, R. E.

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AB - OBJECTIVE: This study was intended to test the hypothesis that glycosylated hemoglobin adversely affects endothelium-dependent vascular relaxation during pregnancy. STUDY DESIGN: Omental artery rings (3 mm) obtained from patients undergoing cesarean delivery at term were suspended in organ chambers for isometric tension recording in Krebs bicarbonate solution. They were bubbled with 5% carbon dioxide in air (37°C, pH 7.4). After equilibration at 1-g passive tension and contraction with endothelin 1, concentration-relaxation curves in response to bradykinin were determined in the presence or absence of oxyhemoglobin, glycosylated hemoglobin, or nitric oxide synthase inhibitors (Nω-nitro-L-arginine methyl ester or Nω-nitro-L- arginine). RESULTS: Oxyhemoglobin and glycosylated hemoglobin attenuated the endothelium-dependent relaxation induced by bradykinin. The inhibition did not differ significantly between the 2 nitric oxide scavengers and was of the same magnitude as that noted with the nitric oxide synthase inhibitors. CONCLUSION: Glycosylation of hemoglobin does not change its effect on endothelium-dependent relaxation in human omental vessels during pregnancy. These data do not support the hypothesis that glycosylated hemoglobin may be the reason for abnormal endothelial function in diabetes.

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