TY - JOUR
T1 - Effect of high-fat diet on peripheral blood mononuclear cells and adipose tissue in early stages of diet-induced weight gain
AU - Lowry, Jake E.
AU - Tumurbaatar, Batbayar
AU - D'agostino, Claudia
AU - Main, Erika
AU - Wright, Traver J.
AU - Dillon, Edgar L.
AU - Saito, Tais
AU - Porter, Craig
AU - Andersen, Clark R.
AU - Brining, Douglas L.
AU - Endsley, Janice J.
AU - Sheffield-Moore, Melinda
AU - Volpi, Elena
AU - Fang, Rong
AU - Abate, Nicola
AU - Tuvdendorj, Demidmaa
N1 - Funding Information:
The present study was supported by the 1KL2TR001441 NIH Training grant, the Institute for Translational Sciences at the UTMB, and in part by a Clinical and Translational Science Award (no. UL1 TR001439) from the National Center for Advancing Translational Sciences, National Institutes of Health, and the Shriners Grant no. 84090, Metabolism Unit, Shriners Hospitals for Children.
Funding Information:
We thank Christopher Danesi, Kathleen Randolph and Anahi D. Delgadillo for help with sample analyses. We thank Dr Sarah Toombs-Smith for the critical editing of this work. The present study was supported by the 1KL2TR001441 NIH Training grant, the Institute for Translational Sciences at the UTMB, and in part by a Clinical and Translational Science Award (no. UL1 TR001439) from the National Center for Advancing Translational Sciences, National Institutes of Health, and the Shriners Grant no. 84090, Metabolism Unit, Shriners Hospitals for Children. D. R. T. was responsible for hypothesis generation and securing funds. J. E. L., C. A., E. M., D. L. B. and D. R. T. conducted the study. B. T., T. J. W., E. L. D., T. B. S., C. P., J. J. E. and D. R. T. analysed the samples. J. E. L., B. T., T. J. W., E. L. D., T. B. S., C. P., J. J. E., M. S.-M., E. V., R. F., N. A. and D. R. T. analysed and interpreted the data. C. R. A. and D. R. T. performed the statistical analyses. All contributors were responsible for manuscript writing, editing and final approval. The authors declare no competing financial interests.
Publisher Copyright:
© The Authors 2019.
PY - 2019/12/28
Y1 - 2019/12/28
N2 - Subcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.
AB - Subcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.
KW - Autophagy
KW - Low-grade inflammation
KW - Obesogenic diet
KW - Peripheral blood mononuclear cells
KW - Subcutaneous adipose tissue
KW - Weight gain
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U2 - 10.1017/S0007114519002472
DO - 10.1017/S0007114519002472
M3 - Article
C2 - 31554524
AN - SCOPUS:85072697872
SN - 0007-1145
VL - 122
SP - 1359
EP - 1367
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 12
ER -