@article{e2b582a4e29940f1a54ba681eb27032b,
title = "Effect of high-fat diet on peripheral blood mononuclear cells and adipose tissue in early stages of diet-induced weight gain",
abstract = "Subcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.",
keywords = "Autophagy, Low-grade inflammation, Obesogenic diet, Peripheral blood mononuclear cells, Subcutaneous adipose tissue, Weight gain",
author = "Lowry, {Jake E.} and Batbayar Tumurbaatar and Claudia D'agostino and Erika Main and Wright, {Traver J.} and Dillon, {Edgar L.} and Saito, {Tais B.} and Craig Porter and Andersen, {Clark R.} and Brining, {Douglas L.} and Endsley, {Janice J.} and Melinda Sheffield-Moore and Elena Volpi and Rong Fang and Nicola Abate and Tuvdendorj, {Demidmaa R.}",
note = "Funding Information: The present study was supported by the 1KL2TR001441 NIH Training grant, the Institute for Translational Sciences at the UTMB, and in part by a Clinical and Translational Science Award (no. UL1 TR001439) from the National Center for Advancing Translational Sciences, National Institutes of Health, and the Shriners Grant no. 84090, Metabolism Unit, Shriners Hospitals for Children. Funding Information: We thank Christopher Danesi, Kathleen Randolph and Anahi D. Delgadillo for help with sample analyses. We thank Dr Sarah Toombs-Smith for the critical editing of this work. The present study was supported by the 1KL2TR001441 NIH Training grant, the Institute for Translational Sciences at the UTMB, and in part by a Clinical and Translational Science Award (no. UL1 TR001439) from the National Center for Advancing Translational Sciences, National Institutes of Health, and the Shriners Grant no. 84090, Metabolism Unit, Shriners Hospitals for Children. D. R. T. was responsible for hypothesis generation and securing funds. J. E. L., C. A., E. M., D. L. B. and D. R. T. conducted the study. B. T., T. J. W., E. L. D., T. B. S., C. P., J. J. E. and D. R. T. analysed the samples. J. E. L., B. T., T. J. W., E. L. D., T. B. S., C. P., J. J. E., M. S.-M., E. V., R. F., N. A. and D. R. T. analysed and interpreted the data. C. R. A. and D. R. T. performed the statistical analyses. All contributors were responsible for manuscript writing, editing and final approval. The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} The Authors 2019.",
year = "2019",
month = dec,
day = "28",
doi = "10.1017/S0007114519002472",
language = "English (US)",
volume = "122",
pages = "1359--1367",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "12",
}